ArticleAdvanced biosystems2020
Dynamic Click Hydrogels for Xeno-Free Culture of Induced Pluripotent Stem Cells.
Article in Advanced biosystems, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Dynamic Gelatin Hydrogels Crosslinked by Dithiolane-Norbornene Click Chemistry.Macromolecular bioscience · 2026Article
- Norbornene Homopolymerization Limits Cell Spreading in Thiol-Ene Photoclick Hydrogels.Advanced healthcare materials · 2025Article
- Design and Characterization of Thioester Networks with Adaptable and Enzymatically Degradable Cross-Links.Macromolecules · 2025Article
- Click Hydrogels to Assess Stiffness-Induced Activation of Pancreatic Cancer-Associated Fibroblasts and Its Impact on Cancer Cell Spreading.Chembiochem : a European journal of chemical biology · 2025Article
- Aqueous Synthesis of Poly(ethylene glycol)-amide-Norbornene-Carboxylate for Modular Hydrogel Crosslinking.Advanced materials interfaces · 2025Article
- Impact of Annealing Chemistry on the Properties and Performance of Microporous Annealed Particle Hydrogels.Biomacromolecules · 2024Article
- Soft Polyethylene Glycol Hydrogels Support Human PSC Pluripotency and Morphogenesis.ACS biomaterials science & engineering · 2024Article
- Degradable and Multifunctional PEG-Based Hydrogels Formed by iEDDA Click Chemistry with Stable Click-Induced Supramolecular Interactions.Macromolecules · 2024Article
- Photo-click hydrogels for 3D in situ differentiation of pancreatic progenitors from induced pluripotent stem cells.Stem cell research & therapy · 2023Article
- Modeling development using hydrogels.Development (Cambridge, England) · 2023Article
- Poly(ethylene glycol)-Norbornene as a Photoclick Bioink for Digital Light Processing 3D Bioprinting.ACS applied materials & interfaces · 2023Article
- Hydrolytically Degradable PEG-Based Inverse Electron Demand Diels-Alder Click Hydrogels.ACS biomaterials science & engineering · 2022Article
- Heparinized Gelatin-Based Hydrogels for Differentiation of Induced Pluripotent Stem Cells.Biomacromolecules · 2022Article
- Dual Functionalization of Gelatin for Orthogonal and Dynamic Hydrogel Cross-Linking.ACS biomaterials science & engineering · 2021Article
- Biomimetic stiffening of cell-laden hydrogels via sequential thiol-ene and hydrazone click reactions.Acta biomaterialia · 2021Article
- Facile Synthesis of Rapidly Degrading PEG-Based Thiol-Norbornene Hydrogels.ACS macro letters · 2021Article
- Hydrogel Models with Stiffness Gradients for Interrogating Pancreatic Cancer Cell Fate.Bioengineering (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Xeno-free, chemically defined poly(ethylene glycol) (PEG)-based hydrogels are being increasingly used for in vitro culture and differentiation of human induced pluripotent stem cells (hiPSCs). These synthetic matrices provide tunable gelation and adaptable material properties crucial for guiding stem cell fate. Here, sequential norbornene-click chemistries are integrated to form synthetic, dynamically tunable PEG-peptide hydrogels for hiPSCs culture and differentiation. Specifically, hiPSCs are photoencapsulated in thiol-norbornene hydrogels crosslinked by multiarm PEG-norbornene (PEG-NB) and proteaselabile crosslinkers. These matrices are used to evaluate hiPSC growth under the influence of extracellular matrix properties. Tetrazine-norbornene (Tz-NB) click reaction is then employed to dynamically stiffen the cell-laden hydrogels. Fast reactive Tz and its stable derivative methyltetrazine (mTz) are tethered to multiarm PEG, yielding mono-functionalized PEG-Tz, PEG-mTz, and dualfunctionalized PEG-Tz/mTz that react with PEG-NB to form additional crosslinks in the cell-laden hydrogels. The versatility of Tz-NB stiffening is demonstrated with different Tz-modified macromers or by intermittent incubation of PEG-Tz for temporal stiffening. Finally, the Tz-NB-mediated dynamic stiffening is explored for 4D culture and definitive endoderm differentiation of hiPSCs. Overall, this dynamic hydrogel platform affords exquisite controls of hydrogel crosslinking for serving as a xeno-free and dynamic stem cell niche.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.