ArticleBiomedical optics express2020
Waveguide-based surface-enhanced Raman spectroscopy detection of protease activity using non-natural aromatic amino acids.
Article in Biomedical optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Targeted detection ofJournal of medical microbiology · 2025Article
- Numerical Analysis of a SiN Digital Fourier Transform Spectrometer for a Non-Invasive Skin Cancer Biosensor.Sensors (Basel, Switzerland) · 2025Article
- Single-Walled Carbon Nanotube Probes for Protease Characterization Directly in Cell-Free Expression Reactions.Analytical chemistry · 2025Article
- Single-Walled Carbon Nanotube Probes for Protease Characterization Directly in Cell-Free Expression Reactions.bioRxiv : the preprint server for biology · 2025Article
- Non-Canonical Amino Acids in Analyses of Protease Structure and Function.International journal of molecular sciences · 2023Review
- Waveguide-Enhanced Raman Spectroscopy (WERS): An Emerging Chip-Based Tool for Chemical and Biological Sensing.Sensors (Basel, Switzerland) · 2022Review
- Translation of a Protease Turnover Assay for Clinical Discrimination of Mucinous Pancreatic Cysts.Diagnostics (Basel, Switzerland) · 2022Article
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Authors and funding
9 authors.
Funding
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Abstract
Surface enhanced Raman spectroscopy (SERS) is a selective and sensitive technique, which allows for the detection of protease activity by monitoring the cleavage of peptide substrates. Commonly used free-space based SERS substrates, however, require the use of bulky and expensive instrumentation, limiting their use to laboratory environments. An integrated photonics approach aims to implement various free-space optical components to a reliable, mass-reproducible and cheap photonic chip. We here demonstrate integrated SERS detection of trypsin activity using a nanoplasmonic slot waveguide as a waveguide-based SERS substrate. Despite the continuously improving SERS performance of the waveguide-based SERS substrates, they currently still do not reach the SERS enhancements of free-space substrates. To mitigate this, we developed an improved peptide substrate in which we incorporated the non-natural aromatic amino acid 4-cyano-phenylalanine, which provides a high intrinsic SERS signal. The use of non-natural aromatics is expected to extend the possibilities for multiplexing measurements, where the activity of several proteases can be detected simultaneously.
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Registered trials
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