ArticleBiomedical optics express2020
zOPT: an open source optical projection tomography system and methods for rapid 3D zebrafish imaging.
Article in Biomedical optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed.
- Reconstructing Three-Dimensional Optical Anisotropy with Tomographic Müller-Polarimetric Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- An Experimental Platform for Tomographic Reconstruction of Tissue Images in Brightfield Microscopy.Sensors (Basel, Switzerland) · 2023Article
- Article
- Chondroitin/dermatan sulfate glycosyltransferase genes are essential for craniofacial development.PLoS genetics · 2022Article
- Dynamics of the Zebrafish Skeleton in Three Dimensions During Juvenile and Adult Development.Frontiers in physiology · 2022Article
- Frame localisation optical projection tomography.Scientific reports · 2021Article
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optical projection tomography (OPT) is a 3D imaging alternative to conventional microscopy which allows imaging of millimeter-sized object with isotropic micrometer resolution. The zebrafish is an established model organism and an important tool used in genetic and chemical screening. The size and optical transparency of the embryo and larva makes them well suited for imaging using OPT. Here, we present an open-source implementation of an OPT platform, built around a customized sample stage, 3D-printed parts and open source algorithms optimized for the system. We developed a versatile automated workflow including a two-step image processing approach for correcting the center of rotation and generating accurate 3D reconstructions. Our results demonstrate high-quality 3D reconstruction using synthetic data as well as real data of live and fixed zebrafish. The presented 3D-printable OPT platform represents a fully open design, low-cost and rapid loading and unloading of samples. Our system offers the opportunity for researchers with different backgrounds to setup and run OPT for large scale experiments, particularly in studies using zebrafish larvae as their key model organism.
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Registered trials
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