ArticleInternational journal of clinical and experimental pathology2020
Correlations of SDF-1 and CXCR4 levels with caspase-3 expression in the retina of rats after optic nerve injury.
Article in International journal of clinical and experimental pathology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed, 1 citations in OpenAlex.
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
objectiveThis study explores the correlations of stromal cell-derived factor-1 (SDF-1) and CXC chemokine receptor 4 (CXCR4) levels with caspase-3 expression in the retina of rats after optic nerve injury. MATERIALS AND
methodsA total of 24 adult healthy specific pathogen-free Sprague-Dawley rats were selected and randomly divided into an optic nerve injury group (n=16) and an optic nerve sham-injury group (n=8). The optic nerve injury group was further sub-divided into a 3 d group (n=8) and a 7 d group (n=8) after their injuries. In the optic nerve injury group, the left eye of each rat was removed and prepared for the optic nerve injury model using the optic nerve clamping method. In the sham-injury group, the optic nerve in the left eye was only exposed without being clamped. The rats were sacrificed at 3 d and 7 d after their optic nerve injuries, and the retina was isolated. The expressions of SDF-1, CXCR4, and caspase-3 in the retina of the rats in each group were measured using an immunohistochemical method. Messenger ribonucleic acid (mRNA) and the protein expressions of SDF-1, CXCR4, and caspase-3 (cleaved caspase-3) in the retinas of rats were measured using quantitative real-time polymerase chain reaction (qPCR) and western blotting, respectively. Moreover, the correlations of the expression of SDF-1 and CXCR4 with caspase-3 expression were analyzed using the Spearman method. The apoptosis of retinal ganglion cells of rats in each group was observed using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining.
resultsImmunohistochemistry of the retinas revealed that, compared with those in the sham-injury group, the expressions of SDF-1 and CXCR4 in the retina in the 3 d group and the 7 d group were gradually increased. Caspase-3 expression was significantly elevated at 3 d after the injuries, but obviously decreased at 7 d after the injuries. The results of qPCR showed that the relative expression levels of SDF-1 and CXCR4 mRNA in the retina at 3 d and 7 d after optic nerve injuries were also significantly higher than those in the sham-injury group (
conclusionAfter optic nerve injury, the continuous increase of the SDF-1 and CXCR4 levels suppresses the apoptosis of retinal cells and repairs the retina by inhibiting the cleavage activation of caspase-3. This provides new insights for the ongoing treatment of optic nerve injury.
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Identifiers
32922601PMC7476942W3086296459What OpenQuestion holds
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