Evidence map›Paper›PMID 32922374›Full record

ArticleFrontiers in microbiology2020

Development of a New Internally Controlled One-Step Real-Time RT-PCR for the Molecular Detection of Enterovirus A71 in Africa and Madagascar.

Romain Volle, Marie-Line Joffret, Kader Ndiaye, Maria Dolores Fernandez-Garcia, Richter Razafindratsimandresy, Jean-Michel Heraud, Dorra Rezig, Serge Alain Sadeuh-Mba, Leila Boulahbal-Anes, Mohamed Seghier and 3 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Comprehensive Review on Viral RNA Extraction Strategies for Enhanced Molecular Diagnostics.Interdisciplinary perspectives on infectious diseases · 2025
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 8 institutions in 7 countries.

Romain VolleInstitut Pasteur, Unité de Biologie des Virus Entériques, Paris, France.
Marie-Line JoffretInstitut Pasteur, Unité de Biologie des Virus Entériques, Paris, France.
Kader NdiayeInstitut Pasteur de Dakar, Dakar, Senegal.
Maria Dolores Fernandez-GarciaInstitut Pasteur de Dakar, Dakar, Senegal.
Richter RazafindratsimandresyInstitut Pasteur de Madagascar, Antananarivo, Madagascar.
Jean-Michel HeraudInstitut Pasteur de Madagascar, Antananarivo, Madagascar.
Dorra RezigInstitut Pasteur de Tunis, Tunis, Tunisia.
Serge Alain Sadeuh-MbaCentre Pasteur du Cameroun, Yaoundé, Cameroon.
Leila Boulahbal-AnesInstitut Pasteur d'Algérie, Algiers, Algeria.
Mohamed SeghierInstitut Pasteur d'Algérie, Algiers, Algeria.
Jagadish M DeshpandehNational Institute of Virology, Indian Council of Medical Research (ICMR), Mumbai, India.
Maël BessaudInstitut Pasteur, Unité de Biologie des Virus Entériques, Paris, France.
Francis DelpeyrouxInstitut Pasteur, Unité de Biologie des Virus Entériques, Paris, France.
Inserm · FRInstitut Pasteur · FRInstitut Pasteur d'Algérie · DZInstitut Pasteur de Dakar · SNInstitut Pasteur de Madagascar · MGCentre Pasteur du Cameroun · CMICMR-National Institute of Virology · INInstitut Pasteur de Tunis · TN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enterovirus A71 (EV-A71) is a leading cause of hand-foot-and-mouth disease (HFMD) and can be associated with severe neurological complications. EV-A71 strains can be classified into seven genogroups, A-H, on the basis of the VP1 capsid protein gene sequence. Genogroup A includes the prototype strain; genogroups B and C are responsible of major outbreaks worldwide, but little is known about the others, particularly genogroups E and F, which have been recently identified in Africa and Madagascar, respectively. The circulation of EV-A71 in the African region is poorly known and probably underestimated. A rapid and specific assay for detecting all genogroups of EV-A71 is required. In this study, we developed a real-time RT-PCR assay with a competitive internal control (IC). The primers and

Indexed as

enterovirus 71EV-A71HFMDmolecular detectionnew genogroups

Identifiers

PMID32922374
PMCPMC7456875
OpenAlexW3049763945

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.