ArticleChemical physics letters2019
A fraction of NADH in solution is "dark": Implications for metabolic sensing via fluorescence lifetime.
Article in Chemical physics letters, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Agmatine as the initiating substrate for aspartate semialdehyde-dependent bacterial spermidine biosynthesis.The Journal of biological chemistry · 2026Article
- Submicromolar imaging of intrinsic chromophores by two-photon photothermal microscopy captures mitochondrial response to chemotherapy.Science advances · 2026Article
- AI-Powered Mining of Highly Customized and Superior ESIPT-Based Fluorescent Probes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding.Advanced drug delivery reviews · 2023Review
- Ultrafast fluorescence dynamics of NADH in aprotic solvents: Quasi-static self-quenching unmasked.Journal of photochemistry and photobiology. A, Chemistry · 2023Article
- Ultrafast Fluorescence Spectroscopy via Upconversion and Its Applications in Biophysics.Molecules (Basel, Switzerland) · 2021Review
- Dehydrogenase Binding Sites Abolish the "Dark" Fraction of NADH: Implication for Metabolic Sensing via FLIM.The journal of physical chemistry. B · 2020Article
- Femtosecond Fluorescence Spectra of NADH in Solution: Ultrafast Solvation Dynamics.The journal of physical chemistry. B · 2020Article
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Authors and funding
11 authors.
Funding
Abstract
The metabolic cofactor and energy carrier NADH (nicotinamide adenine dinucleotide, reduced) has fluorescence yield and lifetime that depends strongly on conformation, a fact that has enabled metabolic monitoring of cells via FLIM (Fluorescence Lifetime Microscopy). Using femtosecond fluorescence upconversion, we show that this molecule in solution participates in ultrafast self-quenching along with both bulk solvent relaxation and spectral relaxation on 1.4 and 26 ps timescales. This, in effect, means up to a third of NADH is effectively "dark" for FLIM in the 400-500 nm observation window commonly employed. Methods to compensate for, avoid or measure dark species corrections are outlined.
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