Evidence map›Paper›PMID 32920606›Full record

ArticlePediatric research2021

Intestinal resection affects whole-body arginine synthesis in neonatal piglets.

Marihan Lansing, George Slim, Pamela Wizzard, Mahroukh Rafii, Paul B Pencharz, Patrick N Nation, Megan R Beggs, R Todd Alexander, Paul W Wales, Justine M Turner and 1 more

Abstract read
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In one paragraph

Article in Pediatric research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Marihan LansingDepartment of Pediatrics, University of Alberta, Edmonton, AB, Canada.
George SlimDepartment of Pediatrics, University of Alberta, Edmonton, AB, Canada.
Pamela WizzardDepartment of Pediatrics, University of Alberta, Edmonton, AB, Canada.
Mahroukh RafiiResearch Institute, The Hospital for Sick Children, Toronto, ON, Canada.
Paul B PencharzDepartments of Pediatrics and Nutritional Sciences, University of Toronto, Toronto, ON, Canada.
Patrick N NationLaboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.
Megan R BeggsDepartment of Physiology at the University of Alberta, Edmonton, AB, Canada.
R Todd AlexanderDepartment of Pediatrics, University of Alberta, Edmonton, AB, Canada.
Paul W WalesDepartment of Pediatrics, University of Alberta, Edmonton, AB, Canada.
Justine M TurnerDepartment of Pediatrics, University of Alberta, Edmonton, AB, Canada. Justine.turner@albertahealthservices.ca.
Ron O BallDepartment of Agricultural, Life & Environmental Sciences, University of Alberta, Edmonton, AB, Canada.
University of Alberta · CAHospital for Sick Children · CAUniversity of Toronto · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPrevious studies in piglets show a direct relationship between intestinal mass and arginine (Arg) synthesis. We aimed to study the effects of 75% intestinal resection on whole-body Arg synthesis.

methodsPiglets were allocated to sham or jejunocolic (JC) surgery and to enteral nutrition (EN) at 20% [sham (n = 8), JC (n = 10)], or 40% [sham (n = 4), JC (n = 5)]. A gastric tube was placed for EN and a venous catheter for parenteral nutrition and blood sampling. On day 6, a primed bolus and constant infusion of Arg m + 2 label and proline m + 1 label was delivered. In addition, 40% EN piglets received a citrulline (Cit) m + 3 tracer. Blood sampling was undertaken and whole-body Arg synthesis was calculated. On day 7, intestinal length was measured, and samples were collected for gene expression (PCR quantification) and histopathology.

resultsOn Day 7, sham piglets showed intestinal lengthening compared to JC (p = 0.02). Whole-body Arg synthesis was similar between groups (p = 0.50). Adjusting for absolute small intestinal length, JC piglets had greater Arg synthesis (p = 0.01). Expression of arginosuccinase was upregulated in the jejunum of JC compared to sham on 20% EN (p = 0.03).

conclusionThis demonstrates for the first-time adaptive changes in intestinal Arg synthesis following intestinal resection. IMPACT: The intestine makes a critical contribution to whole-body arginine synthesis, particularly in neonates, a human population at risk for short bowel syndrome. Therefore, we studied intestinal arginine synthesis in a neonatal piglet model of short bowel syndrome and demonstrated adaptive changes in the intestine that may preserve whole-body arginine synthesis, despite loss of intestinal mass. This research adds new information to our understanding of the effects a massive intestinal resection has on amino acid metabolism during neonatal development.

Indexed as

Animals, NewbornAnimalsArginineDisease Models, AnimalIntestinesMaleSwineArginine

Identifiers

PMID32920606
OpenAlexW3085134782

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.