ArticleClinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases2020
Sample pooling for SARS-CoV-2 RT-PCR screening.
Article in Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Pathogens on High-Touch Surfaces in an Arid Megacity: A Longitudinal Molecular Surveillance Study.Microorganisms · 2026Article
- Performance Evaluation of Nasopharyngeal Swab Pooling to Improve SARS-CoV-2 Testing Capacity via Closed Real-Time RT‒PCR Assays.Infection and drug resistance · 2026Article
- Evaluation of sample pooling using Xpert Carba-R and XpertMicrobiology spectrum · 2025Article
- Article
- Evaluation of Fecal Sample Pooling for Real-Time RT-PCR Testing SARS-CoV-2 in Animals.Viruses · 2024Article
- Semi-quantitative group testing for efficient and accurate qPCR screening of pathogens with a wide range of loads.BMC bioinformatics · 2024Article
- Evaluations of modes of pooling specimens for COVID-19 screened by quantitative PCR and droplet digital PCR.Scientific reports · 2024Article
- A novel strategy to avoid sensitivity loss in pooled testing for SARS-CoV-2 surveillance: validation using nasopharyngeal swab and saliva samples.Frontiers in public health · 2023Article
- A Pooling Strategy for Detecting Carbapenem Resistance Genes by the Xpert Carba-R Test in Rectal Swab Specimens.Journal of clinical microbiology · 2022Article
- Multicenter study evaluating novel multi-specimen pooling assay for the detection of SARS-CoV-2: High sensitivity and high throughput testing.Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi · 2022Article
- Singleplex, multiplex and pooled sample real-time RT-PCR assays for detection of SARS-CoV-2 in an occupational medicine setting.Scientific reports · 2022Article
- Digital PCR Applications in the SARS-CoV-2/COVID-19 Era: a Roadmap for Future Outbreaks.Clinical microbiology reviews · 2022Review
- A four specimen-pooling scheme reliably detects SARS-CoV-2 and influenza viruses using the BioFire FilmArray Respiratory Panel 2.1.Scientific reports · 2022Article
- Improvement of SARS-COV-2 screening using pooled sampling testing in limited RT-qPCR resources.Journal of virological methods · 2022Article
- Prevalence of SARS-CoV-2 and co-infection with malaria during the first wave of the pandemic (the Burkina Faso case).Frontiers in public health · 2022Article
- SARS-CoV-2 detection in multi-sample pools in a real pandemic scenario: A screening strategy of choice for active surveillance.PloS one · 2022Article
- Evaluation of Sample Pooling for SARS-CoV-2 Detection in Nasopharyngeal Swab and Saliva Samples with the Idylla SARS-CoV-2 Test.Microbiology spectrum · 2021Article
- Testing individual and pooled saliva samples for sars-cov-2 nucleic acid: a prospective study.Diagnostic microbiology and infectious disease · 2021Article
- An accurate model for SARS-CoV-2 pooled RT-PCR test errors.Royal Society open science · 2021Article
- Pooled Testing Strategies for SARS-CoV-2 diagnosis: A comprehensive review.Diagnostic microbiology and infectious disease · 2021Review
Corrections and comments
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Authors and funding
27 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo evaluate the efficacy of sample pooling compared to the individual analysis for the diagnosis of coronavirus disease 2019 (COVID-19) by using different commercial platforms for nucleic acid extraction and amplification.
methodsA total of 3519 nasopharyngeal samples received at nine Spanish clinical microbiology laboratories were processed individually and in pools (342 pools of ten samples and 11 pools of nine samples) according to the existing methodology in place at each centre.
resultsWe found that 253 pools (2519 samples) were negative and 99 pools (990 samples) were positive; with 241 positive samples (6.85%), our pooling strategy would have saved 2167 PCR tests. For 29 pools (made out of 290 samples), we found discordant results when compared to their correspondent individual samples, as follows: in 22 of 29 pools (28 samples), minor discordances were found; for seven pools (7 samples), we found major discordances. Sensitivity, specificity and positive and negative predictive values for pooling were 97.10% (95% confidence interval (CI), 94.11-98.82), 100%, 100% and 99.79% (95% CI, 99.56-99.90) respectively; accuracy was 99.80% (95% CI, 99.59-99.92), and the kappa concordant coefficient was 0.984. The dilution of samples in our pooling strategy resulted in a median loss of 2.87 (95% CI, 2.46-3.28) cycle threshold (C
conclusionsWe found a high efficiency of pooling strategies for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA testing across different RNA extraction and amplification platforms, with excellent performance in terms of sensitivity, specificity and positive and negative predictive values.
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