ArticleMolecular and cellular probes2020
Establishment of a duplex SYBR green I-based real-time polymerase chain reaction assay for the rapid detection of canine circovirus and canine astrovirus.
Article in Molecular and cellular probes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Review
- Canine circovirus: emergence, adaptation, and challenges for animal and public health.Frontiers in veterinary science · 2025Review
- First Report on the Molecular Detection of Canine Astrovirus (CaAstV) in Dogs with Gastrointestinal Disease in Ecuador Using a Fast and Sensitive RT-qPCR Assay Based on SYBR GreenVeterinary sciences · 2024Article
- Molecular characterization and lineage analysis of canine astrovirus strains from dogs with gastrointestinal disease in Ecuador based on ORF-2 gene analysis.Frontiers in veterinary science · 2024Article
- Development of a Real-time TaqMan PCR Assay for The Detection of Porcine Circovirus 4.Journal of veterinary research · 2022Article
- Reconstruction of the Evolutionary Origin, Phylodynamics, and Phylogeography of the Porcine Circovirus Type 3.Frontiers in microbiology · 2022Article
- Establishment of hydrolysis probe system real-time PCR assay for rapid detection of canine circovirus.3 Biotech · 2021Article
- Anti-protozoal potential of electrospun polymeric nanofiber composite membranes for treatment of contaminated drinking water.Pathogens and global healthArticle
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The similar clinical characteristics of canine circovirus (CaCV) and canine astrovirus (CaAstV) infections and high frequency of co-infection make diagnosis difficult. In this study, a duplex SYBR Green I-based real-time polymerase chain reaction (PCR) assay was established for the rapid, simultaneous detection of CaCV and CaAstV. Two pairs of specific primers were designed based on the Rep gene of CaCV and the Cap gene of CaAstV. By using the real-time PCR assay method, the two viruses can be distinguished by the difference in melting temperatures, 79 °C and 86 °C for CaCV and CaAstV, respectively. This assay had high specificity, showing no cross-reaction with other common canine viruses, as well as high sensitivity, with minimum detection limits of 9.25 × 10
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