Evidence map›Paper›PMID 32918396›Full record

ArticleChemMedChem2020

A Cytochrome c-Chlorotoxin Hybrid Protein as a Possible Antiglioma Drug.

Louis J Delinois, Harold Peón, Juan C Villalobos-Santos, Josell Ramírez-Paz, Jennifer Miller, Kai H Griebenow, Arthur D Tinoco

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ChemMedChem, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Louis J DelinoisChemistry Department, Faculty of Natural Sciences of the University of Puerto Rico at Río Piedras, San Juan, Puerto Rico, 00925, USA.
Harold PeónAcademia del Perpetuo Socorro High School, San Juan, Puerto Rico, 00907, USA.
Juan C Villalobos-SantosBiology Department, Faculty of Natural Sciences of the University of Puerto Rico at Río Piedras, San Juan, Puerto Rico, 00925, USA.
Josell Ramírez-PazChemistry Department, Faculty of Natural Sciences of the University of Puerto Rico at Río Piedras, San Juan, Puerto Rico, 00925, USA.
Jennifer MillerChemistry Department, Faculty of Natural Sciences of the University of Puerto Rico at Río Piedras, San Juan, Puerto Rico, 00925, USA.
Kai H GriebenowChemistry Department, Faculty of Natural Sciences of the University of Puerto Rico at Río Piedras, San Juan, Puerto Rico, 00925, USA.
Arthur D TinocoChemistry Department, Faculty of Natural Sciences of the University of Puerto Rico at Río Piedras, San Juan, Puerto Rico, 00925, USA.ORCID 0000-0003-2825-5235
University of Puerto Rico at Río Piedras · PR

Funding

Upgrade Laboratories Facilities at UPR-Rio PiedrasR25GM061151 · NIGMS · UNIVERSITY OF PUERTO RICO RIO PIEDRAS · PI CARBALLEIRA, NESTOR MANUEL · 2001 to 2023
$32.5M
The Role of Sensory Input to Mammalian Locomotion After the Loss of supraspinal.P20GM103642 · NIGMS · UNIVERSITY OF PUERTO RICO MED SCIENCES · PI LASALDE-DOMINICCI, JOSE ANTONIO · 2013 to 2023
$20.6M
Journey: NIDDK Short-Term Education Program for Underrepresented Persons/STEP-UPR25DK098067 · NIDDK · UNIVERSITY OF WISCONSIN-MADISON · PI DODGE FRANCIS, CAROLEE · 2012 to 2021
$1.8M
Development of Peptide-Conjugate Biomimics for Targeted Ti(IV)-Based Anticancer DSC1CA190504 · NCI · UNIVERSITY OF PUERTO RICO RIO PIEDRAS · PI TINOCO, ARTHUR DAVID · 2014 to 2017
$1.4M
NCI NIH HHS SC1 CA190504NIDDK NIH HHS R25 DK098067NIGMS NIH HHS P20 GM103642NIGMS NIH HHS R25 GM061151
6 · The paper itself

Abstract

Malignant gliomas are the most lethal form of primary brain tumors. Despite advances in cancer therapy, the prognosis of glioma patients has remained poor. Cytochrome c (Cytc), an endogenous heme-based protein, holds tremendous potential to treat gliomas because of its innate capacity to trigger apoptosis. To this end, a hybrid cytochrome c-chlorotoxin (Cytc-CTX) protein was biosynthesized to enable cellular uptake of the cell impenetrable Cytc using CTX transporters. A nucleotide sequence containing 1 : 1 Cytc and CTX was constructed and separated by a hexahistidine-tag and an enterokinase cleavage site. The sequence was cloned into a pBTR1 plasmid, expressed in Escherichia coli, purified via 2-dimensional chromatography. The identity and size of the protein were determined by Western blot and mass spectrometry. Cytc in this soluble hybrid protein has similar structure and stability as human Cytc and the hybrid protein is endocytosed into a glioma cell line, while displaying potent cytotoxicity and a favorable therapeutic index. Its facile, low-cost, and high yield synthesis, biocompatibility, and robustness suggest that the hybrid protein is a promising candidate for antiglioma drug evaluation.

Indexed as

Antineoplastic AgentsBrain NeoplasmsCells, CulturedCell SurvivalCytochromes cDose-Response Relationship, DrugGliomaHumansScorpion VenomsStructure-Activity RelationshipAntineoplastic AgentsChlorotoxinCytochromes cScorpion Venomsantiglioma drugapoptosiscytochrome c-chlorotoxinmetal-based biomoleculerecombinant hybrid protein

Identifiers

PMID32918396
PMCPMC7722079
OpenAlexW3086994685

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.