ArticleScientific reports2020
Three-dimensional alignment of microvasculature and cardiomyocytes in the developing ventricle.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- A Simple and Fast Optical Clearing Method for Whole-Mount Fluorescence In Situ Hybridization (FISH) Imaging.Journal of biophotonics · 2025Article
- Digital labeling for 3D histology: segmenting blood vessels without a vascular contrast agent using deep learning.Biomedical optics express · 2023Article
- Three-dimensional visualization of heart-wide myocardial architecture and vascular network simultaneously at single-cell resolution.Frontiers in cardiovascular medicine · 2022Article
- Facile Fabrication of Three-Dimensional Hydrogel Film with Complex Tissue Morphology.Bioengineering (Basel, Switzerland) · 2021Article
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Authors and funding
5 authors.
Funding
Abstract
While major coronary artery development and pathologies affecting them have been extensively studied, understanding the development and organization of the coronary microvasculature beyond the earliest developmental stages requires new tools. Without techniques to image the coronary microvasculature over the whole heart, it is likely we are underestimating the microvasculature's impact on normal development and diseases. We present a new imaging and analysis toolset to visualize the coronary microvasculature in intact embryonic hearts and quantify vessel organization. The fluorescent dyes DiI and DAPI were used to stain the coronary vasculature and cardiomyocyte nuclei in quail embryo hearts during rapid growth and morphogenesis of the left ventricular wall. Vessel and cardiomyocytes orientation were automatically extracted and quantified, and vessel density was calculated. The coronary microvasculature was found to follow the known helical organization of cardiomyocytes in the ventricular wall. Vessel density in the left ventricle did not change during and after compaction. This quantitative and automated approach will enable future cohort studies to understand the microvasculature's role in diseases such as hypertrophic cardiomyopathy where misalignment of cardiomyocytes has been observed in utero.
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