ArticleParticle and fibre toxicology2020
Carbon nanomaterial-derived lung burden analysis using UV-Vis spectrophotometry and proteinase K digestion.
Article in Particle and fibre toxicology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Environmentally relevant aged nanoplastics amplify oxidative stress-associated inhalation toxicity and delay lung clearance.Redox biology · 2026Article
- Pulmonary toxicity of brake wear particles: effects of composition and surface reactivity.Particle and fibre toxicology · 2026Article
- Ultraviolet irradiation-induced enhancement of inflammatory potential of polystyrene nano- and microplastics and effects of dispersion on lung clearance.Particle and fibre toxicology · 2026Article
- Simulated aquatic aging exacerbates pulmonary toxicity of graphite nanoplatelets by mechanically and chemically induced surface oxidation, enhancing oxidative potential.Particle and fibre toxicology · 2026Article
- A simple and reliable method for the qualitative and quantitative analysis of nano- and microplastics in organs for controlled preclinical studies.Particle and fibre toxicology · 2026Article
- In vitro dosimetry of multi-walled carbon nanotubes on human bronchial epithelial cells (BEAS-2B) using UV-Vis-NIR spectrophotometry and chemical digestion.Analytical and bioanalytical chemistry · 2026Article
- Biomimetic Carbon-Based Nanomaterials: From Design Strategies to Next-Generation Biosensing and Theranostic Applications.Small (Weinheim an der Bergstrasse, Germany) · 2025Review
- Ferrocene-derived magnetic fiber-particles from diesel exhaust: enhanced pulmonary toxicity via Bach1-SAT1-polyamine depletion.Journal of nanobiotechnology · 2025Article
- Multimodal pulmonary clearance kinetics of carbon black nanoparticles deposited in the lungs of rats: the role of alveolar macrophages.Particle and fibre toxicology · 2024Article
- Effect of spParticle and fibre toxicology · 2023Article
- High-efficiency mechanically assisted alkaline extraction of nanoparticles from biological tissues for spICP-MS analysis.Analytical and bioanalytical chemistry · 2022Article
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5 authors.
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Abstract
backgroundThe quantification of nanomaterials accumulated in various organs is crucial in studying their toxicity and toxicokinetics. However, some types of nanomaterials, including carbon nanomaterials (CNMs), are difficult to quantify in a biological matrix. Therefore, developing improved methodologies for quantification of CNMs in vital organs is instrumental in their continued modification and application.
resultsIn this study, carbon black, nanodiamond, multi-walled carbon nanotube, carbon nanofiber, and graphene nanoplatelet were assembled and used as a panel of CNMs. All CNMs showed significant absorbance at 750 nm, while their bio-components showed minimal absorbance at this wavelength. Quantification of CNMs using their absorbance at 750 nm was shown to have more than 94% accuracy in all of the studied materials. Incubating proteinase K (PK) for 2 days with a mixture of lung tissue homogenates and CNMs showed an average recovery rate over 90%. The utility of this method was confirmed in a murine pharyngeal aspiration model using CNMs at 30 μg/mouse.
conclusionsWe developed an improved lung burden assay for CNMs with an accuracy > 94% and a recovery rate > 90% using PK digestion and UV-Vis spectrophotometry. This method can be applied to any nanomaterial with sufficient absorbance in the near-infrared band and can differentiate nanomaterials from elements in the body, as well as the soluble fraction of the nanomaterial. Furthermore, a combination of PK digestion and other instrumental analysis specific to the nanomaterial can be applied to organ burden analysis.
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