Evidence map›Paper›PMID 32917195›Full record

ArticleBMC veterinary research2020

Removal of bovine digital dermatitis-associated treponemes from hoof knives after foot-trimming: a disinfection field study.

A V Gillespie, S D Carter, R W Blowey, G J Staton, N J Evans

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.9field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. The Presence ofMicroorganisms · 2021
    Article
  6. Review
  7. Lesion Material FromFrontiers in veterinary science · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

A V GillespieDepartment of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Merseyside, UK. amyg@liverpool.ac.uk.ORCID http://orcid.org/0000-0002-0017-3388
S D CarterDepartment of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Merseyside, UK.
R W BloweyWood Veterinary Group, 125 Bristol Road, Gloucester, GL2 4NB, UK.
G J StatonDepartment of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Merseyside, UK.
N J EvansDepartment of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Merseyside, UK.
University of Liverpool · GB

Funding

Agricultural and Horticultural Development Board 148388Biotechnology and Biological Sciences Research Council BB/M011186/1
6 · The paper itself

Abstract

backgroundBovine digital dermatitis (BDD) is an infectious foot disease found commonly in dairy herds. Foot-trimming is an important husbandry procedure for reducing the ensuing lameness; however, epidemiological, and microbiological studies have identified this as a risk activity for transmitting BDD. Three disinfectants have previously been identified in laboratory work as effective for removing viable BDD-associated Treponema spp., from hoof knife blades. The present study enrolled 133 dairy cattle with BDD lesions, and swabbed hoof knife blades before and after foot-trimming, and after knife disinfection with one of three disinfectants (1:100 FAM30®, 2% Virkon® and 2% sodium hypochlorite) to assess their efficacy under field conditions.

resultsDetection of BDD treponeme phylogroup DNA was undertaken by direct PCR of swabs, and viable treponemes were detected by PCR of swab cultures after 6 weeks' incubation. Where hoof knives did not contact the lesion, BDD-associated treponemes were detected after foot-trimming in 12/22 (54.5%) cases by direct PCR and 1/22 (4.5%) cases by PCR of cultured organisms. Where contact was made with the lesion, 111/111 (100%) samples taken after trimming were positive by direct PCR and 47/118 (39.8%) were positive by culture PCR. Viable organisms were identified in cultures from lesion stages M2, M3, M4 and M4.1. No viable organisms were detected after disinfection of hoof knives.

conclusionsHoof knives post-trimming were frequently contaminated with BDD-associated treponeme DNA. Viable organisms were identified in cultures whether contact had been made between hoof knife and lesion or not, although contact clearly increased the frequency of detection of viable organisms. The three disinfectants tested were effective for removing viable organisms. The disinfection protocol used in this study should therefore be considered reliable for adoption as standard industry practice.

Indexed as

AnimalsCattleCattle DiseasesDairyingDigital DermatitisDisinfectantsDisinfectionDNA, BacterialEquipment ContaminationFemaleHoof and ClawIodophorsPeroxidesSodium HypochloriteSulfuric AcidsTreponemaDisinfectantsDNA, BacterialIodophorsmonoperoxysulfatePeroxidesSodium HypochloriteSulfuric AcidsBovineDairy cattleDigital dermatitisDisinfectionFoot-trimmingHoof knivesTreponemes

Identifiers

PMID32917195
PMCPMC7488572
OpenAlexW3085275326

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.