Evidence map›Paper›PMID 32916256›Full record

ArticleGenomics2020

Genomic insights of Klebsiella pneumoniae isolated from a native Amazonian fish reveal wide resistome against heavy metals, disinfectants, and clinically relevant antibiotics.

Louise Cerdeira, Daniel F M Monte, Bruna Fuga, Fábio P Sellera, Ingrith Neves, Larissa Rodrigues, Mariza Landgraf, Nilton Lincopan

Open access · hybridAbstract read
In one paragraph

Article in Genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.7field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. The mobilome landscape of biocide-resistance in Brazilian ESKAPE isolates.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024
    Article
  5. Review
  6. Article
  7. Review
  8. Genomic insights into ESBL-producingFrontiers in veterinary science · 2023
    Article
  9. Beta-Lactam-ResistantPathogens (Basel, Switzerland) · 2022
    Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Louise CerdeiraDepartment of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil; Department of Infectious Diseases, Central Clinical School, Monash University, Australia; One Health Brazilian Resistance Project (OneBR), Brazil. Electronic address: louise.cerdeira@monash.edu.
Daniel F M MonteOne Health Brazilian Resistance Project (OneBR), Brazil; Department of Food and Experimental Nutrition, Food Research Center, Faculty of Pharmaceutical Sciences, University of São Paulo, Brazil.
Bruna FugaDepartment of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil; One Health Brazilian Resistance Project (OneBR), Brazil; Department of Microbiology, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.
Fábio P SelleraOne Health Brazilian Resistance Project (OneBR), Brazil; Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Ingrith NevesDepartment of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil; One Health Brazilian Resistance Project (OneBR), Brazil.
Larissa RodriguesDepartment of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil; One Health Brazilian Resistance Project (OneBR), Brazil.
Mariza LandgrafDepartment of Food and Experimental Nutrition, Food Research Center, Faculty of Pharmaceutical Sciences, University of São Paulo, Brazil.
Nilton LincopanDepartment of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil; One Health Brazilian Resistance Project (OneBR), Brazil; Department of Microbiology, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil. Electronic address: lincopan@usp.br.
Universidade de São Paulo · BRMonash University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A multidrug-resistant CTX-M-15-producing Klebsiella pneumoniae (KpP1 strain) was isolated from a native Amazonian fish (Brachyplatystoma filamentosum) at the Brazilian Amazon. The strain was identified by MALDI-TOF. The genome was extracted, purified and a Nextera DNA Flex library was prepared and sequenced by Illumina platform. The sequenced genome was de novo assembled using Unicycler and in silico prediction accomplished by curated bioinformatics tools. The size of the genome is 5.6 Mb with 5715 genes. Whole-genome sequencing analysis revealed the presence of wide resistome, with genes conferring resistance to clinically relevant antibiotics, heavy metals and disinfectants. The KpP1 strain was assigned to the sequence type ST3827, KL111 (wzi113) and O3b locus. Native freshwater fish sold in wet markets of the Amazonian region could be an important vehicle for transmission of multidrug-resistant bacteria to humans. This study may give genomic insights on the spread of critical-priority WHO pathogens in a One Health context.

Indexed as

Drug Resistance, Multiple, BacterialGenome, BacterialAnimalsAnti-Bacterial AgentsBrazilCatfishesDisinfectantsGenomicsKlebsiella pneumoniaeMetals, HeavyAnti-Bacterial AgentsDisinfectantsMetals, HeavyEnterobacteralesESBLFoodqnrE1Resistome

Identifiers

PMID32916256
PMCPMC7758709
OpenAlexW3084015354

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.