ArticlePloS one2020
Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- One Embryology: impactful connections between human- and wildlife-assisted reproduction.Journal of assisted reproduction and genetics · 2026Review
- Sperm energy-restriction and recovery enhances equine sperm function and embryo development after ICSI.Journal of animal science · 2026Article
- Article
- Current and Emerging Advanced Techniques for Breeding Donkeys and Mules.Animals : an open access journal from MDPI · 2025Review
- 40 'wild' years: the current reality and future potential of assisted reproductive technologies in wildlife species.Animal reproduction · 2024Review
- In Vitro-Produced Equine Blastocysts Exhibit Greater Dispersal and Intermingling of Inner Cell Mass Cells than In Vivo Embryos.International journal of molecular sciences · 2023Article
- Postmortem Collection of Gametes for the Conservation of Endangered Mammals: A Review of the Current State-of-the-Art.Animals : an open access journal from MDPI · 2023Review
- First sex modification case in equine cloning.PloS one · 2023Article
- Oocyte Penetration Speed Optimization Based on Intracellular Strain.Micromachines · 2022Article
- Evaluation of fertilization capability of frozen-thawed completely immotile spermatozoa collected from a white bengal tiger after interspecific ICSI with bovine oocytes.Animal reproduction · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several equids have gone extinct and many extant equids are currently considered vulnerable to critically endangered. This work aimed to evaluate whether domestic horse oocytes support preimplantation development of zebra embryos obtained by intracytoplasmic sperm injection (ICSI, zebroid) and cloning, and to study the Hippo signaling pathway during the lineage specification of trophectoderm cells and inner cell mass cells. We first showed that zebra and horse sperm cells induce porcine oocyte activation and recruit maternal SMARCA4 during pronuclear formation. SMARCA4 recruitment showed to be independent of the genetic background of the injected sperm. No differences were found in blastocyst rate of ICSI hybrid (zebra spermatozoon into horse egg) embryos relative to the homospecific horse control group. Interestingly, zebra cloned blastocyst rate was significantly higher at day 8. Moreover, most ICSI and cloned horse and zebra blastocysts showed a similar expression pattern of SOX2 and nuclear YAP1 with the majority of the nuclei positive for YAP1, and most SOX2+ nuclei negative for YAP1. Here we demonstrated that horse oocytes support zebra preimplantation development of both, ICSI and cloned embryos, without compromising development to blastocyst, blastocyst cell number neither the expression of SOX2 and YAP1. Our results support the use of domestic horse oocytes as a model to study in vitro zebra embryos on behalf of preservation of valuable genetic.
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