ArticleInternational journal of legal medicine2021
Postmortem expression of apoptosis-related genes in the liver of mice and their use for estimation of the time of death.
Article in International journal of legal medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 2 of them syntheses that pooled it.
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Who cites it
9 citing papers in PubMed, 2 syntheses or guidelines pooled it, 20 citations in OpenAlex.
- Potential Role of mRNA in Estimating Postmortem Interval: A Systematic Review.International journal of molecular sciences · 2024Pooled it
- A small RNA, microRNA as a potential biomolecular marker to estimate post mortem interval in forensic science: a systematic review.International journal of legal medicine · 2023Pooled it
- Temporal RT-qPCR-Based Porcine Cardiac Molecular Profiling for Post-Mortem Interval Estimation: Predictive Modeling.International journal of molecular sciences · 2026Article
- Postmortem interval estimation based on protein analysis and marker studies in different organs in vivo.Forensic science, medicine, and pathology · 2025Article
- UPLC/Q-TOF MS-based femoral muscle metabolomic analysis under high-temperature: a proof of concept for postmortem interval estimation.Frontiers in medicine · 2025Article
- Short-term postmortem interval estimation by detection of apoptosis-related protein in skin.Forensic science, medicine, and pathology · 2024Article
- Combined metabolomics and tandem machine-learning models for wound age estimation: a novel analytical strategy.Forensic sciences research · 2023Article
- Caspase 9 and Caspase 3 Immunohistochemical Pattern in Skeletal and Cardiac Muscles at Different Times after Death: An Experimental Study on PMI Estimation.Diagnostics (Basel, Switzerland) · 2021Article
- Cadaveric Stem Cells: Their Research Potential and Limitations.Frontiers in genetics · 2021Review
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1 author at 1 institution in 1 country.
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Abstract
purposeA major challenge in forensic medicine is to estimate the postmortem interval (PMI). Several approaches had been tried to determine the time of death, including physical and chemical changes. This study aims to explore the postmortem changes in the expression of apoptosis-related genes in the liver of mice and to use these changes for estimation of the PMI.
methodsHepatic tissue was collected from sacrificed mice immediately after death (the control group) and at 3, 6, 9, 12, 18, and 24 hours after death. Four apoptosisrelated genes were selected as target genes, which are Caspase 3 (Casp3), B cell leukemia/ lymphoma 2 (Bcl2), BCL2-associated X protein (Bax), and Transformation related protein 53 (Trp53), and their relative expression was measured using quantitative PCR. miR-122 was used as a reference gene for normalization of the Ct (threshold cycle) values of the target genes.
resultsThe results revealed that the postmortem expression of Casp3 increased in a time-dependent manner; the expression of Bax increased from 3 to 18 hours followed by a decrease at 24 hours after death; the expression of Bcl2 decreased in a time-dependent manner after death; the expression of Trp53 increased from 3 to 6 hours and then started to decrease from 9 to 24 hours after death.
conclusionBased on the observed changes in the expression level of these genes, mathematical models were established to estimate the PMI. Further research is needed to investigate these markers and mathematical models in human tissues.
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