Evidence map›Paper›PMID 32913981›Full record

ArticleJCO precision oncology2018

Multiplex Gene Profiling of Cell-Free DNA in Patients With Metastatic Melanoma for Monitoring Disease.

Selena Y Lin, Sharon K Huang, Kelly T Huynh, Matthew P Salomon, Shu-Ching Chang, Diego M Marzese, Richard B Lanman, AmirAli Talasaz, Dave S B Hoon

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JCO precision oncology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Selena Y Lin, , , , , and , John Wayne Cancer Institute at Providence Saint John's Health Center, Santa Monica; and , Guardant Health, Redwood City, CA; and , Medical Data Research Center at Providence Saint Joseph's Health, Portland, OR.
Sharon K Huang, , , , , and , John Wayne Cancer Institute at Providence Saint John's Health Center, Santa Monica; and , Guardant Health, Redwood City, CA; and , Medical Data Research Center at Providence Saint Joseph's Health, Portland, OR.
Kelly T Huynh, , , , , and , John Wayne Cancer Institute at Providence Saint John's Health Center, Santa Monica; and , Guardant Health, Redwood City, CA; and , Medical Data Research Center at Providence Saint Joseph's Health, Portland, OR.
Matthew P Salomon, , , , , and , John Wayne Cancer Institute at Providence Saint John's Health Center, Santa Monica; and , Guardant Health, Redwood City, CA; and , Medical Data Research Center at Providence Saint Joseph's Health, Portland, OR.
Shu-Ching Chang, , , , , and , John Wayne Cancer Institute at Providence Saint John's Health Center, Santa Monica; and , Guardant Health, Redwood City, CA; and , Medical Data Research Center at Providence Saint Joseph's Health, Portland, OR.
Diego M Marzese, , , , , and , John Wayne Cancer Institute at Providence Saint John's Health Center, Santa Monica; and , Guardant Health, Redwood City, CA; and , Medical Data Research Center at Providence Saint Joseph's Health, Portland, OR.
Richard B Lanman, , , , , and , John Wayne Cancer Institute at Providence Saint John's Health Center, Santa Monica; and , Guardant Health, Redwood City, CA; and , Medical Data Research Center at Providence Saint Joseph's Health, Portland, OR.
AmirAli Talasaz, , , , , and , John Wayne Cancer Institute at Providence Saint John's Health Center, Santa Monica; and , Guardant Health, Redwood City, CA; and , Medical Data Research Center at Providence Saint Joseph's Health, Portland, OR.
Dave S B Hoon, , , , , and , John Wayne Cancer Institute at Providence Saint John's Health Center, Santa Monica; and , Guardant Health, Redwood City, CA; and , Medical Data Research Center at Providence Saint Joseph's Health, Portland, OR.

Funding

Identification of Melanoma Brain Metastasis Tumor BiomarkersR01CA167967 · NCI · SAINT JOHN'S CANCER INSTITUTE · PI HOON, DAVE S B · 2013 to 2017
$1.9M
NCI NIH HHS R01 CA167967
6 · The paper itself

Abstract

purposeHotspot blood cell-free DNA (cfDNA) biomarker assays have limited utility in profiling tumor heterogeneity and burden and in capturing regional metastasis with low disease burden in patients with melanoma. We investigated the utility of a sensitive 54-cancer gene digital next-generation sequencing approach targeting blood cfDNA single nucleotide variants (SNVs) and copy number amplification for monitoring disease in patients with melanoma with regional or distant organ metastasis (DOM). PATIENTS AND

methodsA total of 142 blood samples were evaluated by digital next-generation sequencing across two patient cohorts. Cohort 1 contained 44 patients with stage II, III, or IV disease with matched tumor DNA at the time of surgery or DOM. Cohort 2 consisted of 12 overlapping patients who were longitudinally monitored after complete lymph node dissection to DOM.

resultsIn cohort 1, cfDNA SNVs were detected in 75% of patients. Tumor-cfDNA somatic SNV concordance was 85% at a variant allele fraction of ≥ 0.5%. An SNV load (number of unique SNVs detected) of greater than two SNVs and an SNV burden (total cumulative SNV VAF) of > 0.5% were significantly associated with worse overall survival (

conclusionThis study demonstrates significant utility for cfDNA profiling in patients with melanoma with regional and/or distant metastasis for earlier detection of recurrence and progression and in capturing tumor evolution and heterogeneity, thus impacting how patients with melanoma are monitored.

Identifiers

PMID32913981
PMCPMC7446321

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.