ArticleScientific reports2020
Evolution of isoprenyl diphosphate synthase-like terpene synthases in fungi.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 24 citations in OpenAlex.
- Divergent Lineage of Terpene Synthases Establishes Terpenoid Biosynthesis in Brown Macroalgae.Journal of the American Chemical Society · 2026Article
- Expansion of the geranylgeranyl pyrophosphate synthase gene family underlies the evolution of terpenoid biosynthesis in termites.PLoS biology · 2026Article
- Discovery and Biosynthesis of Farnesyl Pyrophosphate-Derived Noncanonical CJournal of the American Chemical Society · 2026Article
- Characterizing and decoding the effects of co-culture fermentation on dark tea infusion flavor development: elucidating microbial succession and metabolic dynamics mediated byFood chemistry: X · 2025Article
- Canonical terpene synthases in arthropods: Intraphylum gene transfer.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Terpene biosynthesis in marine sponge animals.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Functional characterization of a farnesyl diphosphate synthase from Dendrobium nobile Lindl.AMB Express · 2022Article
- Biocompatible fluorocarbon liquid underlays forRSC advances · 2022Article
- Systematic mining of fungal chimeric terpene synthases using an efficient precursor-providing yeast chassis.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Terpenoid Biosynthesis Dominates among Secondary Metabolite Clusters inJournal of fungi (Basel, Switzerland) · 2021Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Terpene synthases (TPSs) and trans-isoprenyl diphosphate synthases (IDSs) are among the core enzymes for creating the enormous diversity of terpenoids. Despite having no sequence homology, TPSs and IDSs share a conserved "α terpenoid synthase fold" and a trinuclear metal cluster for catalysis, implying a common ancestry with TPSs hypothesized to evolve from IDSs anciently. Here we report on the identification and functional characterization of novel IDS-like TPSs (ILTPSs) in fungi that evolved from IDS relatively recently, indicating recurrent evolution of TPSs from IDSs. Through large-scale bioinformatic analyses of fungal IDSs, putative ILTPSs that belong to the geranylgeranyl diphosphate synthase (GGDPS) family of IDSs were identified in three species of Melampsora. Among the GGDPS family of the two Melampsora species experimentally characterized, one enzyme was verified to be bona fide GGDPS and all others were demonstrated to function as TPSs. Melampsora ILTPSs displayed kinetic parameters similar to those of classic TPSs. Key residues underlying the determination of GGDPS versus ILTPS activity and functional divergence of ILTPSs were identified. Phylogenetic analysis implies a recent origination of these ILTPSs from a GGDPS progenitor in fungi, after the split of Melampsora from other genera within the class of Pucciniomycetes. For the poplar leaf rust fungus Melampsora larici-populina, the transcripts of its ILTPS genes were detected in infected poplar leaves, suggesting possible involvement of these recently evolved ILTPS genes in the infection process. This study reveals the recurrent evolution of TPSs from IDSs since their ancient occurrence and points to the possibility of a wide distribution of ILTPS genes in three domains of life.
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