ArticleScientific reports2020
Catalytic specificity of the Lactobacillus plantarum cystathionine γ-lyase presumed by the crystallographic analysis.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 27 citations in OpenAlex.
- Family-Specialized Transformer for L-cystathionine gamma-lyase Engineering and Its Structural Interpretation.Computational and structural biotechnology journal · 2026Article
- Molecular basis for the diversification of lincosamide biosynthesis by pyridoxal phosphate-dependent enzymes.Nature chemistry · 2025Article
- Elucidation of the stereochemical mechanism of cystathionine γ-lyase reveals how substrate specificity constrains catalysis.ACS catalysis · 2024Article
- pH-dependent regulation of an acidophilicApplied and environmental microbiology · 2024Article
- Catalytic specificity and crystal structure of cystathionine γ-lyase from Pseudomonas aeruginosa.Scientific reports · 2024Article
- Cystathionine γ-Lyase Self-Inactivates by Polysulfidation during Cystine Metabolism.International journal of molecular sciences · 2023Article
- Mechanism-based and computational modeling of hydrogen sulfide biogenesis inhibition: interfacial inhibition.Scientific reports · 2023Article
- Structural basis of the inhibition of cystathionine γ-lyase from Toxoplasma gondii by propargylglycine and cysteine.Protein science : a publication of the Protein Society · 2023Article
- Article
- An insight into gut microbiota and metabolites in the mice with adenomyosis.Frontiers in cellular and infection microbiology · 2023Article
- Role of Hydrogen Sulfide in Oral Disease.Oxidative medicine and cellular longevity · 2022Review
- Molecular Responses of Lactobacilli to Plant Phenolic Compounds: A Comparative Review of the Mechanisms Involved.Antioxidants (Basel, Switzerland) · 2021Review
- Physical Exercise Repairs Obstructive Jaundice-Induced Damage to Intestinal Mucosal Barrier FunctionFrontiers in physiology · 2021Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The reverse transsulfuration pathway, which is composed of cystathionine β-synthase (CBS) and cystathionine γ-lyase (CGL), plays a role to synthesize L-cysteine using L-serine and the sulfur atom in L-methionine. A plant-derived lactic acid bacterium Lactobacillus plantarum SN35N has been previously found to harbor the gene cluster encoding the CBS- and CGL-like enzymes. In addition, it has been demonstrated that the L. plantarum CBS can synthesize cystathionine from O-acetyl-L-serine and L-homocysteine. The aim of this study is to characterize the enzymatic functions of the L. plantarum CGL. We have found that the enzyme has the high γ-lyase activity toward cystathionine to generate L-cysteine, together with the β-lyase activity toward L-cystine to generate L-cysteine persulfide. By the crystallographic analysis of the inactive CGL K194A mutant complexed with cystathionine, we have found the residues which recognize the distal amino and carboxyl groups of cystathionine or L-cystine. The PLP-bound substrates at the active site may take either the binding pose for the γ- or β-elimination reaction, with the former being the major reaction in the case of cystathionine.
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