Evidence map›Paper›PMID 32912097›Full record

ArticleStroke2020

Sex and Genetic Background Effects on the Outcome of Experimental Intracranial Aneurysms.

Takeshi Yanagisawa, Hua Zhang, Tomoaki Suzuki, Yoshinobu Kamio, Tsubasa Takizawa, Andreia Morais, David Y Chung, Tao Qin, Yuichi Murayama, James E Faber and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stroke, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 2 pooled it
0.7field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 2 syntheses or guidelines pooled it, 17 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Genetic determinants of insufficiency of the collateral circulation.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 6 institutions in 3 countries.

Takeshi YanagisawaNeurovascular Research Laboratory, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown (T.Y., T.S., T.T., D.Y.C., T.Q., A.M., C.A.).
Hua ZhangDepartment of Cell Biology and Physiology, University of North Carolina, Chapel Hill. (H.Z., J.E.F.).
Tomoaki SuzukiNeurovascular Research Laboratory, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown (T.Y., T.S., T.T., D.Y.C., T.Q., A.M., C.A.).
Yoshinobu KamioDepartment of Neurosurgery, Hamamatsu University School of Medicine, Japan (Y.K.).
Tsubasa TakizawaNeurovascular Research Laboratory, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown (T.Y., T.S., T.T., D.Y.C., T.Q., A.M., C.A.).
Andreia MoraisNeurovascular Research Laboratory, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown (T.Y., T.S., T.T., D.Y.C., T.Q., A.M., C.A.).
David Y ChungNeurovascular Research Laboratory, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown (T.Y., T.S., T.T., D.Y.C., T.Q., A.M., C.A.).
Tao QinNeurovascular Research Laboratory, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown (T.Y., T.S., T.T., D.Y.C., T.Q., A.M., C.A.).
Yuichi MurayamaDepartment of Neurosurgery, Jikei University School of Medicine, Tokyo, Japan (T.Y., Y.M.).
James E FaberDepartment of Cell Biology and Physiology, University of North Carolina, Chapel Hill. (H.Z., J.E.F.).
Aman B PatelDepartment of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston. (T.Y., A.B.P.).
Cenk AyataNeurovascular Research Laboratory, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown (T.Y., T.S., T.T., D.Y.C., T.Q., A.M., C.A.).
Harvard University · USMassachusetts General Hospital · USJikei University School of Medicine · JPUniversity of North Carolina at Chapel Hill · USHamamatsu University School of Medicine · JPTwitter (United States) · US

Funding

Institutional Career Development CoreKL2TR002542 · NCATS · HARVARD MEDICAL SCHOOL · PI BREDELLA, MIRIAM ANTOINETTE, RUTKOVE, SEWARD B. · 2018 to 2022
$8.7M
Non-invasive vagus nerve stimulation targeting cortical spreading depression in migrane prophylaxisR01NS102969 · NINDS · MASSACHUSETTS GENERAL HOSPITAL · PI AYATA, CENK · 2017 to 2021
$1.9M
Determinants of Functional Brain Connectivity After Subarachnoid HemorrhageK08NS112601 · NINDS · MASSACHUSETTS GENERAL HOSPITAL · PI CHUNG, DAVID YOUNG · 2019 to 2023
$1.0M
NCATS NIH HHS KL2 TR002542NINDS NIH HHS K08 NS112601NINDS NIH HHS R01 NS102969
6 · The paper itself

Abstract

background and purposeIntracranial aneurysm formation and rupture risk are, in part, determined by genetic factors and sex. To examine their role, we compared 3 mouse strains commonly used in cerebrovascular studies in a model of intracranial aneurysm formation and rupture.

methodsIntracranial aneurysms were induced in male CD1 (Crl:CD1[ICR]), male and female C57 (C57BL/6NCrl), and male 129Sv (129S2/SvPasCrl or 129S1/SvImJ) mice by stereotaxic injection of elastase at the skull base, combined with systemic deoxycorticosterone acetate-salt hypertension. Neurological deficits and mortality were recorded. Aneurysms and subarachnoid hemorrhage grades were quantified postmortem, either after spontaneous mortality or at 7 to 21 days if the animals survived. In separate cohorts, we examined proinflammatory mediators by quantitative reverse transcriptase-polymerase chain reaction, arterial blood pressure via the femoral artery, and the circle of Willis by intravascular latex casting.

resultsWe found striking differences in aneurysm formation, rupture, and postrupture survival rates among the groups. 129Sv mice showed the highest rates of aneurysm rupture (80%), followed by C57 female (36%), C57 male (27%), and CD1 (21%). The risk of aneurysm rupture and the presence of unruptured aneurysms significantly differed among all 3 strains, as well as between male and female C57. The same hierarchy was observed upon Kaplan-Meier analysis of both overall survival and deficit-free survival. Subarachnoid hemorrhage grades were also more severe in 129Sv. CD1 mice showed the highest resistance to aneurysm rupture and the mildest outcomes. Higher mean blood pressures and the major phenotypic difference in the circle of Willis anatomy in 129Sv provided an explanation for the higher incidence of and more severe aneurysm ruptures. TNFα (tumor necrosis factor-alpha), IL-1β (interleukin-1-beta), and CCL2 (chemokine C-C motif ligand 2) expressions did not differ among the groups.

conclusionsThe outcome of elastase-induced intracranial aneurysm formation and rupture in mice depends on genetic background and shows sexual dimorphism.

Indexed as

Genetic BackgroundAneurysm, RupturedAnimalsDesoxycorticosteroneDisease Models, AnimalFemaleIntracranial AneurysmMaleMiceMice, Inbred C57BLMice, Inbred ICRPancreatic ElastaseSex FactorsSurvival RateDesoxycorticosteronePancreatic Elastaseaneurysmgeneticshypertensionmodels, animalsubarachnoid hemorrhage

Identifiers

PMID32912097
PMCPMC7530050
OpenAlexW3084638496

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.