ArticleBiotechnology letters2021
Temperature-controlled molecular weight of hyaluronic acid produced by engineered Bacillus subtilis.
Article in Biotechnology letters, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 25 citations in OpenAlex.
- Molecular weight regulation strategies in microbial synthesis of hyaluronic acid: current status and future perspectives.World journal of microbiology & biotechnology · 2025Review
- Hyaluronic Acid and Its Synthases-Current Knowledge.International journal of molecular sciences · 2025Review
- Starvation-induced changes in the proteome and transcriptome of the salivary glands of leech (Hirudo nipponia).PloS one · 2024Article
- Advances in Gelatin Bioinks to Optimize Bioprinted Cell Functions.Advanced healthcare materials · 2023Review
- Microbial Hyaluronic Acid Production: A Review.Molecules (Basel, Switzerland) · 2023Review
- Review
- Low cost and sustainable hyaluronic acid production in a manufacturing platform based on Bacillus subtilis 3NA strain.Applied microbiology and biotechnology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
To produce high-, medium- and low-molecular-weight hyaluronic acid (HA) at different temperatures using engineered Bacillus subtilis expressing hyaluronidase (HAase) from leech. By overexpressing the HAase gene hya in the HA-producing strain WmB using temperature-sensitive plasmid pKSV7, the engineered strain WmB-PYh produced HA with different molecular weights (8.61 kDa at 32 °C, 0.615 MDa at 42 °C, and 6.19 MDa at 47 °C). In this study, the molecular weight of HA was regulated by using leech HAase expressed from a temperature-sensitive plasmid. We thus obtained different molecular weight HAs by using a single bacterial strain at different culture temperatures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.