ArticleMolecular biology reports2020
Different miRNA contents between mammary epithelial cells and milk fat globules: a random or a targeted process?
Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 19 citations in OpenAlex.
- Characterization of mammary glands and milk fat globule transcripts in lactating buffalo and goats.Food chemistry. Molecular sciences · 2025Article
- Extracellular vesicle miRNome during subclinical mastitis in dairy cows.Veterinary research · 2024Article
- Milk fat miRNome changes in response to LPS challenge in Holstein cows.Veterinary research · 2023Article
- MiRNome variations in milk fractions during feed restrictions of different intensities in dairy cows.BMC genomics · 2023Article
- Comprehensive Profiling of ceRNA (circRNA-miRNA-mRNA) Networks in Hypothalamic-Pituitary-Mammary Gland Axis of Dairy Cows under Heat Stress.International journal of molecular sciences · 2023Article
- Perspective: Milk microRNAs as Important Players in Infant Physiology and Development.Advances in nutrition (Bethesda, Md.) · 2021Article
- Nutritional Regulation of Mammary Gland Development and Milk Synthesis in Animal Models and Dairy Species.Genes · 2021Review
- Progress in epigenetic regulation of milk synthesis, with particular emphasis on mRNA regulation and DNA methylation.Cell cycle (Georgetown, Tex.)Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are small noncoding RNAs present in milk-derived extracellular vesicles and milk fat globules (MFG). Nucleic acid content between the lactating mammary tissue (MT) and MFG are quite similar but discrepancies exist in their miRNA content. Our objective was to identify the origin of these discrepancies, and to evaluate the existence of a possible mechanism sorting miRNAs that will or will not be exported from the mammary epithelial cells (MECs) in bovine MFG. miR-125b-5p, miR-126-3p, miR-141-3p, and miR-204-5p, chosen on the basis of their abundance in the MT, were quantified using RT-qPCR in lactating cow MT, MFG, and laser capture-microdissected MECs. Two miRNAs (miR-125b-5p and miR-141-3p) were detected in the MT as well as in MFG and MECs. miR-204-5p was detected only in the MT, suggesting that it is very likely expressed in a cell type other than MECs. miR-126-3p was detected both in the MT and in MECs but not in MFG, suggesting a targeting mechanism for miRNAs in MECs. These results highlights differences in miRNA content between MECs and MFG may be due to a possibly not random mechanism for loading MFG with miRNA cargos that could involve a variable distribution in MECs or a sorting mechanism.
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