Evidence map›Paper›PMID 32909215›Full record

ArticleMolecular biology reports2020

Cellular retinol binding protein 1 transfection reduces proliferation and AKT-related gene expression in H460 non-small lung cancer cells.

Amedeo Ferlosio, Elena Doldo, Sara Agostinelli, Gaetana Costanza, Federica Centofanti, Angelo Sidoni, Augusto Orlandi

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Amedeo Ferlosio *Anatomic Pathology, Department of Biomedicine and Prevention, Tor Vergata University of Rome, Rome, Italy.
Elena Doldo *Anatomic Pathology, Department of Biomedicine and Prevention, Tor Vergata University of Rome, Rome, Italy.
Sara AgostinelliAnatomic Pathology, Department of Biomedicine and Prevention, Tor Vergata University of Rome, Rome, Italy.
Gaetana CostanzaAnatomic Pathology, Department of Biomedicine and Prevention, Tor Vergata University of Rome, Rome, Italy.
Federica CentofantiAnatomic Pathology, Department of Biomedicine and Prevention, Tor Vergata University of Rome, Rome, Italy.
Angelo SidoniDepartment of Experimental Medicine, Section of Anatomic Pathology and Histology, Medical School, University of Perugia, Perugia, Italy.
Augusto OrlandiAnatomic Pathology, Department of Biomedicine and Prevention, Tor Vergata University of Rome, Rome, Italy. orlandi@uniroma2.it.
University of Rome Tor Vergata · ITPoliclinico Tor Vergata · ITSan Gallicano Hospital · ITUniversity of Perugia · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In recent years, new treatments with novel action mechanisms have been explored for advanced non-small cell lung cancer (NSCLC). Retinoids promote cancer cell differentiation and death and their trafficking and action is mediated from specific cytoplasmic and nuclear receptors, respectively. The purpose of this study was to investigate the effect of Cellular retinol binding protein-1 (CRBP-1) transfection in H460 human NSCLC cell line, normally not expressing CRBP-1. H460 cells were transfected by using a vector pTargeT Mammalian expression system carrying the whole sequence of CRBP-1 gene. For proliferation and apoptosis studies, cells were treated with different concentrations of all-trans Retinoic Acid (atRA) and retinol. AKT-related gene expression was analyzed by using western blot and Signosis array and results analysed by one-way analysis of variance (ANOVA) or by t-student test. CRBP-1

Indexed as

ApoptosisCarcinoma, Non-Small-Cell LungCell Line, TumorCell ProliferationDown-RegulationErbB ReceptorsExtracellular Signal-Regulated MAP KinasesForkhead Box Protein O1Gene Expression Regulation, NeoplasticHumansLung NeoplasmsProliferating Cell Nuclear AntigenProto-Oncogene Proteins c-aktRetinol-Binding Proteins, CellularSignal TransductionTOR Serine-Threonine KinasesAKT1 protein, humanAKT2 protein, humanAKT3 protein, humanEGFR protein, humanErbB ReceptorsExtracellular Signal-Regulated MAP KinasesForkhead Box Protein O1FOXO1 protein, humanp27 antigenProliferating Cell Nuclear AntigenProto-Oncogene Proteins c-aktRBP1 protein, humanRetinol-Binding Proteins, CellularTOR Serine-Threonine KinasesTretinoinAKT pathwayCRBP-1Non-small cell lung cancerRetinoids

Identifiers

PMID32909215
PMCPMC7561531
OpenAlexW3083787226

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.