ArticlePlant molecular biology2021
WRKY22 and WRKY25 transcription factors are positive regulators of defense responses in Nicotiana benthamiana.
Article in Plant molecular biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 80 citations in OpenAlex.
- Molecular changes in agroinfiltrated leaves of Nicotiana benthamiana expressing suppressor of silencing P19 and coronavirus-like particles.Plant biotechnology journal · 2026Article
- Article
- Analysis of Non-Coding RNAs and N6-Methyladenosine-Modified Genes in Response to flg22 in Grape Immunity.Plant biotechnology journal · 2025Article
- Comparative transcriptomics of atrazine-tolerant and sensitive alfalfa varieties under atrazine stress.BMC plant biology · 2025Article
- Smi-miRmTERF regulates organelle development, retrograde signaling, secondary metabolism and immunity via targeting a subset of SmmTERFs in Salvia miltiorrhiza.Molecular horticulture · 2025Article
- Exploring associations between metabolites and gene transcripts of common bean (Phaseolus vulgaris L.) in response to rust (Uromyces appendiculatus) infection.BMC plant biology · 2025Article
- Genome-wide identification and characterization of transcription factors involved in defense responses against Sclerotinia sclerotiorum in Brassica juncea.Scientific reports · 2025Article
- Combined transcriptome and metabolome analysis ofFrontiers in plant science · 2025Article
- Insights ofFrontiers in plant science · 2025Article
- The WRKY Family Transcription Factor GmWRKY72 Represses Glyceollin Phytoalexin Biosynthesis in Soybean.Plants (Basel, Switzerland) · 2024Article
- Insights into bs5 resistance mechanisms in pepper against Xanthomonas euvesicatoria through transcriptome profiling.BMC genomics · 2024Article
- Pepper immunity against Ralstonia solanacearum is positively regulated by CaWRKY3 through modulation of different WRKY transcription factors.BMC plant biology · 2024Article
- WGCNA Reveals Hub Genes and Key Gene Regulatory Pathways of the Response of Soybean to Infection byGenes · 2024Article
- Molecular warfare between pathogenic Fusarium oxysporum R1 and host Crocus sativus L. unraveled by dual transcriptomics.Plant cell reports · 2024Article
- Genome-wide characterization of heavy metal-associated isoprenylated plant protein gene family fromFrontiers in plant science · 2024Article
- Transcriptome analysis revealed that AcWRKY75 transcription factor reduced the resistance of kiwifruit toFrontiers in plant science · 2024Article
- BcWRKY22 ActivatesAntioxidants (Basel, Switzerland) · 2023Article
- Loss-of-function mutations in WRKY22 and WRKY25 impair stomatal-mediated immunity and PTI and ETI responses against Pseudomonas syringae pv. tomato.Plant molecular biology · 2023Article
- Induction of distinct plant cell death programs by secreted proteins from the wheat pathogen Zymoseptoria tritici.Scientific reports · 2022Article
- Functional Identification ofBiomolecules · 2022Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
key messageNbWRKY22 and NbWRKY25 are required for full activation of bacteria-associated pattern- and effector-triggered immunity as well as for the response to other non-bacterial defense elicitors. Plants defend themselves against pathogens using a two-layered immune system. Pattern-triggered immunity (PTI) can be activated upon recognition of epitopes from flagellin including flg22. Pseudomonas syringae pv. tomato (Pst) delivers effector proteins into the plant cell to promote host susceptibility. However, some plants express resistance (R) proteins that recognize specific effectors leading to the activation of effector-triggered immunity (ETI). Resistant tomato lines such as Rio Grande-PtoR (RG-PtoR) recognize two Pst effectors, AvrPto and AvrPtoB, and activate ETI through the Pto/Prf protein complex. Using RNA-seq, we identified two tomato WRKY transcription factor genes, SlWRKY22 and SlWRKY25, whose expression is increased during Pst-induced ETI. Silencing of the WRKY25/22 orthologous genes in Nicotiana benthamiana led to a delay in programmed cell death normally associated with AvrPto recognition or several non-bacterial effector/R protein pairs. An increase in disease symptoms was observed in silenced plants infiltrated with Pseudomonas syringae pv. tabaci expressing AvrPto or HopQ1-1. Expression of both tomato WRKY genes is also induced upon treatment with flg22 and callose deposition and cell death suppression assays in WRKY25/22-silenced N. benthamiana plants supported their involvement in PTI. Our results reveal an important role for two WRKYs as positive regulators of plant immunity against bacterial and potentially non-bacterial pathogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.