Evidence map›Paper›PMID 32909140›Full record

ArticleCytotechnology2020

Modified method for effective primary vascular smooth muscle progenitor cell culture from peripheral blood.

Jin-Hee Seong, Yi-Sun Song, Hyun-Woo Joo, In-Hwa Park, Guang-Yin Shen, Na-Kyoung Shin, A-Hyeon Lee, Amy M Kwon, Yonggu Lee, Hyuck Kim and 1 more

Abstract read
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In one paragraph

Article in Cytotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Jin-Hee SeongGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea.
Yi-Sun SongGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea.
Hyun-Woo JooGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea.
In-Hwa ParkGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea.
Guang-Yin ShenDivision of Cardiology, Department of Internal Medicine, Hanyang University College of Medicine, Seoul, South Korea.
Na-Kyoung ShinGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea.
A-Hyeon LeeGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea.
Amy M KwonBiostatistical Consulting and Research Laboratory, Medical Research Collaborating Center, Industry-University Cooperation Foundation, Hanyang University, Seoul, South Korea.
Yonggu LeeDepartment of Internal Medicine, Hanyang University Guri Hospital, Guri, South Korea.
Hyuck KimDepartment of Thoracic Surgery, Hanyang University Seoul Hospital, Seoul, South Korea.
Kyung-Soo KimGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea. kskim@hanyang.ac.kr.ORCID http://orcid.org/0000-0002-0891-1023

Funding

Korea National Institute of Health HI16C0412
6 · The paper itself

Abstract

In previous studies, vascular smooth muscle progenitor cells (vSMPCs) isolated from peripheral blood mononuclear cells (PBMCs) were cultured using medium containing platelet-derived growth factor-BB (PDGF-BB) for 4 weeks. However, this method requires long culture periods of up to 4 weeks and yields low cell counts. Therefore, we proposed the modified method to improve the cell yield and purity and to reduce the cell culture period. PBMCs were isolated from human peripheral blood and cultured by the conventional method using medium containing PDGF-BB alone or the modified method using medium containing PDGF-BB, basic fibroblast growth factor (bFGF), and insulin-transferrin-selenium ITS for 4 weeks. The purity of vSMPCs was analyzed for the expression of a- smooth muscle actin (SMA) by flow cytometry and significantly higher in the modified method than conventional methods at the 1st and 2nd weeks. Also, mRNA expression of a-SMA by real-time PCR was significantly higher in the modified method than conventional method at the 2 weeks. The yield of vSMPCs by trypan blue exclusion assay was significantly higher in the modified method than conventional method at the 1st, 2nd and 3rd weeks. The primary culture using the modified method with PDGF-BB, bFGF, and ITS not only improved cell purity and yield, but also shortened the culture period, compared to the conventional culture method for vSMPCs. The modified method will be a time-saving and useful tool in various studies related to vascular pathology.

Indexed as

Cell culturePBMCsPDGF-BBPeripheral bloodVSMPCs

Identifiers

PMID32909140
PMCPMC7547929

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