Evidence map›Paper›PMID 32908168›Full record

ArticleScientific reports2020

Greenbug (Schizaphis graminum) herbivory significantly impacts protein and phosphorylation abundance in switchgrass (Panicum virgatum).

Prince Zogli, Sophie Alvarez, Michael J Naldrett, Nathan A Palmer, Kyle G Koch, Lise Pingault, Jeffrey D Bradshaw, Paul Twigg, Tiffany M Heng-Moss, Joe Louis and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 12 citations in OpenAlex.

  1. Dynamic Reconfiguration of Switchgrass Proteomes in Response to Rust (International journal of molecular sciences · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Prince Zogli *Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE, 68583-0816, USA.
Sophie Alvarez *Proteomics and Metabolomics Facility, Nebraska Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Michael J Naldrett *Proteomics and Metabolomics Facility, Nebraska Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Nathan A Palmer *Wheat, Sorghum, and Forage Research Unit, USDA-ARS, Lincoln, NE, 68583-0937, USA.
Kyle G KochDepartment of Entomology, University of Nebraska-Lincoln, Lincoln, NE, 68583-0816, USA.
Lise PingaultDepartment of Entomology, University of Nebraska-Lincoln, Lincoln, NE, 68583-0816, USA.
Jeffrey D BradshawDepartment of Entomology, University of Nebraska-Lincoln, Lincoln, NE, 68583-0816, USA.
Paul TwiggBiology Department, University of Nebraska-Kearney, Kearney, NE, 68849, USA.
Tiffany M Heng-MossDepartment of Entomology, University of Nebraska-Lincoln, Lincoln, NE, 68583-0816, USA.
Joe LouisDepartment of Entomology, University of Nebraska-Lincoln, Lincoln, NE, 68583-0816, USA. joelouis@unl.edu.
Gautam SarathDepartment of Entomology, University of Nebraska-Lincoln, Lincoln, NE, 68583-0816, USA. Gautam.Sarath@ars.usda.gov.
University of Nebraska–Lincoln · USAgricultural Research Service · USUniversity of Nebraska at Kearney · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Switchgrass (Panicum virgatum L.) is an important crop for biofuel production but it also serves as host for greenbugs (Schizaphis graminum Rondani; GB). Although transcriptomic studies have been done to infer the molecular mechanisms of plant defense against GB, little is known about the effect of GB infestation on the switchgrass protein expression and phosphorylation regulation. The global response of the switchgrass cultivar Summer proteome and phosphoproteome was monitored by label-free proteomics shotgun in GB-infested and uninfested control plants at 10 days post infestation. Peptides matching a total of 3,594 proteins were identified and 429 were differentially expressed proteins in GB-infested plants relative to uninfested control plants. Among these, 291 and 138 were up and downregulated by GB infestation, respectively. Phosphoproteome analysis identified 310 differentially phosphorylated proteins (DP) from 350 phosphopeptides with a total of 399 phosphorylated sites. These phosphopeptides had more serine phosphorylated residues (79%), compared to threonine phosphorylated sites (21%). Overall, KEGG pathway analysis revealed that GB feeding led to the enriched accumulation of proteins important for biosynthesis of plant defense secondary metabolites and repressed the accumulation of proteins involved in photosynthesis. Interestingly, defense modulators such as terpene synthase, papain-like cysteine protease, serine carboxypeptidase, and lipoxygenase2 were upregulated at the proteome level, corroborating previously published transcriptomic data.

Indexed as

AphidsHerbivoryAnimalsGene Expression Regulation, PlantPanicumPhosphorylationPhotosynthesisPlant ProteinsProteomeTranscriptomePlant ProteinsProteome

Identifiers

PMID32908168
PMCPMC7481182
OpenAlexW3084128590

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.