ArticleJournal of immunology (Baltimore, Md. : 1950)2020
Mapping Human Monoclonal IgE Epitopes on the Major Dust Mite Allergen Der p 2.
Article in Journal of immunology (Baltimore, Md. : 1950), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Pre-clinical allergenicity assessment of IgE epitope-targeted Der p 2 mutants demonstrate potential as hypoallergenic AIT candidates.Frontiers in immunology · 2025Article
- Structural analysis of human IgE monoclonal antibody epitopes on dust mite allergen Der p 2.The Journal of allergy and clinical immunology · 2024Article
- Precision engineering for localization, validation, and modification of allergenic epitopes.The Journal of allergy and clinical immunology · 2024Review
- A Cell-Free Protein Synthesis Platform to Produce a Clinically Relevant Allergen Panel.ACS synthetic biology · 2023Article
- Peptide immunotherapy for aeroallergens.Allergy and asthma proceedings · 2023Article
- IgE and IgG4 Epitopes of Dermatophagoides and Blomia Allergens before and after Sublingual Immunotherapy.International journal of molecular sciences · 2023Article
- Human Monoclonal IgE Antibodies-a Major Milestone in Allergy.Current allergy and asthma reports · 2023Review
- Biological activity of human IgE monoclonal antibodies targeting Der p 2, Fel d 1, Ara h 2 in basophil mediator release assays.Frontiers in immunology · 2023Article
- Unique allergen-specific human IgE monoclonal antibodies derived from patients with allergic disease.Frontiers in allergy · 2023Article
- Determination of immunogenic epitopes in major house dust mite allergen, Der p 2, via nanoallergens.Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology · 2022Article
- Human IgE monoclonal antibody recognition of mite allergen Der p 2 defines structural basis of an epitope for IgE cross-linking and anaphylaxisPNAS nexus · 2022Article
- Identification of an immunodominant IgE epitope of Der p 39, a novel allergen ofThe World Allergy Organization journal · 2022Article
- Der f 38 Is a Novel TLR4-Binding Allergen Related to Allergy Pathogenesis fromInternational journal of molecular sciences · 2021Article
- Review
- Allergens and their associated small molecule ligands-their dual role in sensitization.Allergy · 2021Review
- MiR-154-5p-MCP1 Axis Regulates Allergic Inflammation by Mediating Cellular Interactions.Frontiers in immunology · 2021Article
- Pathogenic Mechanism of Der p 38 as a Novel Allergen Homologous to RipA and RipB Proteins in Atopic Dermatitis.Frontiers in immunology · 2021Article
- Structural Aspects of the Allergen-Antibody Interaction.Frontiers in immunology · 2020Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
IgE Abs drive the symptoms of allergic disease upon cross-linking allergens on mast cells or basophils. If the IgE binding sites on the allergens could be identified, it may be useful for creating new forms of immunotherapy. However, direct knowledge of the human IgE (hIgE) epitopes is limited because of the very low frequency of IgE-producing B cells in blood. A new hybridoma technology using human B cells from house dust mite-allergic patients was used to identify four Der p 2-specific hIgE mAbs. Their relative binding sites were assessed and compared by immunoassays with three previously studied murine IgG mAbs. Immunoassays showed that the recognition of Der p 2 by the first three hIgE was inhibited by a single murine IgG, but the fourth hIgE recognized a different epitope from all the other mAbs. The functional ability of the hIgE that bind different epitopes to cross-link Der p 2 was demonstrated in a mouse model of passive systemic anaphylaxis. Nuclear magnetic resonance analyses of Der p 2 in complex with IgG and IgE Abs were used to identify specific residues in the epitopes. To our knowledge, the combination of immunoassays to distinguish overlapping epitopes and nuclear magnetic resonance analyses to identify specific residues involved in Ab binding provided the first epitope mapping of hIgE mAbs to an allergen. The technologies developed in this study will be useful in high-resolution mapping of human epitopes on other Ags and the design of improved therapeutics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.