ArticleLife science alliance2020
multicrispr: gRNA design for prime editing and parallel targeting of thousands of targets.
Article in Life science alliance, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- DivergenteLife · 2026Article
- High-throughput evaluation of genetic variants with prime editing sensor libraries.Nature biotechnology · 2025Article
- CRISPR-GATE: a one-stop repository and guide to computational resources for genome editing experimentation.Briefings in bioinformatics · 2025Review
- Kingdom-wide CRISPR guide design with ALLEGRO.Nucleic acids research · 2025Article
- CRISPRware: a software package for contextual gRNA library design.BMC genomics · 2025Article
- Advances in CRISPR-Cas9 in lineage tracing of model animals.Animal models and experimental medicine · 2025Review
- Prime Editing of Mouse Primary Neurons.Methods in molecular biology (Clifton, N.J.) · 2025Article
- From bench to bedside: cutting-edge applications of base editing and prime editing in precision medicine.Journal of translational medicine · 2024Review
- CRISPR technologies for genome, epigenome and transcriptome editing.Nature reviews. Molecular cell biology · 2024Review
- How CRISPR Is Revolutionizing the Generation of New Models for Cancer Research.Cold Spring Harbor perspectives in medicine · 2024Review
- Current RNA strategies in treating cardiovascular diseases.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Review
- The Development, Optimization and Future of Prime Editing.International journal of molecular sciences · 2023Review
- Optimized prime editing in monocot plants using PlantPegDesigner and engineered plant prime editors (ePPEs).Nature protocols · 2023Review
- Prime editing for precise and highly versatile genome manipulation.Nature reviews. Genetics · 2023Review
- Cas9-induced nonhomologous recombination inbioRxiv : the preprint server for biology · 2023Article
- Heritable Cas9-induced nonhomologous recombination inmicroPublication biology · 2023Article
- A comprehensive Bioconductor ecosystem for the design of CRISPR guide RNAs across nucleases and technologies.Nature communications · 2022Article
- Improvement of base editors and prime editors advances precision genome engineering in plants.Plant physiology · 2022Review
- Molecular and Computational Strategies to Increase the Efficiency of CRISPR-Based Techniques.Frontiers in plant science · 2022Review
- Precise plant genome editing using base editors and prime editors.Nature plants · 2021Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Targeting the coding genome to introduce nucleotide deletions/insertions via the CRISPR/Cas9 technology has become a standard procedure. It has quickly spawned a multitude of methods such as prime editing, APEX proximity labeling, or homology directed repair, for which supporting bioinformatics tools are, however, lagging behind. New CRISPR/Cas9 applications often require specific gRNA design functionality, and a generic tool is critically missing. Here, we introduce multicrispr, an R/bioconductor tool, intended to design individual gRNAs and complex gRNA libraries. The package is easy to use; detects, scores, and filters gRNAs on both efficiency and specificity; visualizes and aggregates results per target or CRISPR/Cas9 sequence; and finally returns both genomic ranges and sequences of gRNAs. To be generic, multicrispr defines and implements a genomic arithmetic framework as a basis for facile adaptation to techniques recently introduced such as prime editing or yet to arise. Its performance and design concepts such as target set-specific filtering render multicrispr a tool of choice when dealing with screening-like approaches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.