Evidence map›Paper›PMID 32907847›Full record

ArticleDevelopment (Cambridge, England)2020

IRX3/5 regulate mitotic chromatid segregation and limb bud shape.

Hirotaka Tao, Jean-Philippe Lambert, Theodora M Yung, Min Zhu, Noah A Hahn, Danyi Li, Kimberly Lau, Kendra Sturgeon, Vijitha Puviindran, Xiaoyun Zhang and 11 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Technologies, strategies, and cautions when deconvoluting genome-wide association signals: FTO in focus.Obesity reviews : an official journal of the International Association for the Study of Obesity · 2023
    Review
  5. Article
  6. Frontiers in neuroscience · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 5 institutions in 3 countries.

Hirotaka TaoProgram in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.
Jean-Philippe LambertLunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON M5G 1X5, Canada.ORCID 0000-0002-0833-1221
Theodora M YungProgram in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.
Min ZhuDepartment of Mechanical and Industrial Engineering, University of Toronto, ON M5S 3G8, Canada.
Noah A HahnProgram in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.ORCID 0000-0003-4374-3368
Danyi LiProgram in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.
Kimberly LauProgram in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.
Kendra SturgeonProgram in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.
Vijitha PuviindranProgram in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.
Xiaoyun ZhangProgram in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada.
Wuming GongLillehei Heart Institute, University of Minnesota, Minneapolis, MN 55455, USA.ORCID 0000-0002-3147-4028
Xiao Xiao ChenDepartment of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada.
Gregory AndersonMouse Imaging Centre, Hospital for Sick Children, Toronto Centre for Phenogenomics, Department of Medical Biophysics, University of Toronto, Toronto, ON M5T 3H7, Canada.
Daniel J GarryLillehei Heart Institute, University of Minnesota, Minneapolis, MN 55455, USA.
R Mark HenkelmanMouse Imaging Centre, Hospital for Sick Children, Toronto Centre for Phenogenomics, Department of Medical Biophysics, University of Toronto, Toronto, ON M5T 3H7, Canada.
Yu SunDepartment of Mechanical and Industrial Engineering, University of Toronto, ON M5S 3G8, Canada.
Angelo IulianellaDepartment of Medical Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada.
Yasuhiko KawakamiDepartment of Genetics, Cell Biology and Development, Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA.
Anne-Claude GingrasLunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON M5G 1X5, Canada.
Chi-Chung HuiProgram in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada sevan.hopyan@sickkids.ca cchui@sickkids.ca.ORCID 0000-0001-7047-6694
Sevan HopyanProgram in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada sevan.hopyan@sickkids.ca cchui@sickkids.ca.ORCID 0000-0001-7201-8902
University of Toronto · CAHospital for Sick Children · CAUniversity of Minnesota · USDalhousie University · CALunenfeld-Tanenbaum Research Institute · CA

Funding

Genetic Regulation of Progenitor Cells in Appendicular Skeletal DevelopmentR01AR064195 · NIAMS · UNIVERSITY OF MINNESOTA · PI KAWAKAMI, YASUHIKO · 2013 to 2022
$3.3M
Mechanisms that Govern and Promote Cardiovascular RegenerationR01HL148599 · NHLBI · UNIVERSITY OF MINNESOTA · PI GARRY, DANIEL J., GARRY, MARY G · 2019 to 2022
$3.0M
CIHR 137092NHLBI NIH HHS R01 HL148599NIAMS NIH HHS R01 AR064195
6 · The paper itself

Abstract

Pattern formation is influenced by transcriptional regulation as well as by morphogenetic mechanisms that shape organ primordia, although factors that link these processes remain under-appreciated. Here we show that, apart from their established transcriptional roles in pattern formation, IRX3/5 help to shape the limb bud primordium by promoting the separation and intercalation of dividing mesodermal cells. Surprisingly, IRX3/5 are required for appropriate cell cycle progression and chromatid segregation during mitosis, possibly in a nontranscriptional manner. IRX3/5 associate with, promote the abundance of, and share overlapping functions with co-regulators of cell division such as the cohesin subunits SMC1, SMC3, NIPBL and CUX1. The findings imply that IRX3/5 coordinate early limb bud morphogenesis with skeletal pattern formation.

Indexed as

AnimalsBlotting, WesternChromatidsChromosome SegregationFemaleFluorescent Antibody TechniqueHEK293 CellsHomeodomain ProteinsHumansImmunoprecipitationLimb BudsMass SpectrometryMiceMitosisPregnancyReal-Time Polymerase Chain ReactionHomeodomain ProteinsIrx3 protein, mouseIrx5 protein, mouseTranscription FactorsCohesinIroquoisLimb developmentMesodermal cell intercalationsMitotic chromatid segregationMorphogenesis

Identifiers

PMID32907847
PMCPMC7561487
OpenAlexW3084026776

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.