ArticleGenome biology2020
Natural display of nuclear-encoded RNA on the cell surface and its impact on cell interaction.
Article in Genome biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 73 citations in OpenAlex.
- Defining cell surface and glycosylated RNAs.Trends in genetics : TIG · 2026Review
- Article
- Step-by-Step Protocol for In Situ Profiling of RNA Subcellular Localization Using TATA-seq.Bio-protocol · 2026Article
- Lectin-based detection and expression profiling of native glycoRNAs.Scientific reports · 2026Article
- Review
- Deciphering the RNA landscapes on mammalian cell surfaces.Protein & cell · 2026Article
- GlycoRNAdb: a database of glycoRNA sequences, structures, abundance, and glycan information across tissues and cell lines.Nucleic acids research · 2026Article
- Long noncoding RNA regulation of immunity.Nature immunology · 2026Review
- GlycoRNA in cancer immune regulation and progression: biological mechanisms and translational therapeutic prospects.Frontiers in immunology · 2026Review
- RNA on Display: Cell Surface RNA as a Novel Layer of Cellular Regulation.Research (Washington, D.C.) · 2026Review
- Cell surface RNAs define a new interface for immune recognition.Frontiers in immunology · 2026Review
- GlycoRNA: a new frontier in RNA biology.Science China. Life sciences · 2026Article
- The secret life of RNA and lipids.RNA biology · 2025Review
- DNASE1L3 surveils mitochondrial DNA on the surface of distinct mammalian cells.bioRxiv : the preprint server for biology · 2025Article
- GlycoRNA, a novel RNA modification.Discover oncology · 2025Review
- GlycoRNA-L and glycoRNA-S mediate human monocyte adhesion via binding to Siglec-5.Biochimica et biophysica acta. Molecular cell research · 2025Article
- Beyond the Secretory Pathway: New Insights Into Protein Release.Traffic (Copenhagen, Denmark) · 2025Review
- Profiling RNA subcellular localization in situ by TATA-seq.RNA (New York, N.Y.) · 2025Article
- Protein Functional Effector (pfe) Noncoding RNAS Are Identical to Fragments from Various Noncoding RNAs.International journal of molecular sciences · 2025Article
- Sialic acid aptamer and RNA in situ hybridization-mediated proximity ligation assay for spatial imaging of glycoRNAs in single cells.Nature protocols · 2025Review
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundCompared to proteins, glycans, and lipids, much less is known about RNAs on the cell surface. We develop a series of technologies to test for any nuclear-encoded RNAs that are stably attached to the cell surface and exposed to the extracellular space, hereafter called membrane-associated extracellular RNAs (maxRNAs).
resultsWe develop a technique called Surface-seq to selectively sequence maxRNAs and validate two Surface-seq identified maxRNAs by RNA fluorescence in situ hybridization. To test for cell-type specificity of maxRNA, we use antisense oligos to hybridize to single-stranded transcripts exposed on the surface of human peripheral blood mononuclear cells (PBMCs). Combining this strategy with imaging flow cytometry, single-cell RNA sequencing, and maxRNA sequencing, we identify monocytes as the major type of maxRNA+ PBMCs and prioritize 11 candidate maxRNAs for functional tests. Extracellular application of antisense oligos of FNDC3B and CTSS transcripts inhibits monocyte adhesion to vascular endothelial cells.
conclusionsCollectively, these data highlight maxRNAs as functional components of the cell surface, suggesting an expanded role for RNA in cell-cell and cell-environment interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.