Evidence map›Paper›PMID 32907618›Full record

ArticleVeterinary research2020

Construction of polycistronic baculovirus surface display vectors to express the PCV2 Cap(d41) protein and analysis of its immunogenicity in mice and swine.

Ya-Yi Chen, Wei-Chen Yang, Yu-Kang Chang, Chi-Young Wang, Wei-Ru Huang, Jyun-Yi Li, Kuo-Pin Chuang, Hung-Yi Wu, Ching-Dong Chang, Brent L Nielsen and 1 more

Open access · goldAbstract read
In one paragraph

Article in Veterinary research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 19 citations in OpenAlex.

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  8. Surface Display ofFrontiers in immunology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Ya-Yi ChenDepartment of Stomatology, Tung's Taichung MetroHarbor Hospital, Taichung, Taiwan.
Wei-Chen YangInstitute of Molecular Biology, National Chung Hsing University, Taichung, 402, Taiwan.
Yu-Kang ChangDepartment of Medical Research, Tung's Taichung MetroHarbor Hospital, Taichung, Taiwan.
Chi-Young WangDepartment of Veterinary Medicine, National Chung Hsing University, Taichung, 402, Taiwan.
Wei-Ru HuangInstitute of Molecular Biology, National Chung Hsing University, Taichung, 402, Taiwan.
Jyun-Yi LiInstitute of Molecular Biology, National Chung Hsing University, Taichung, 402, Taiwan.
Kuo-Pin ChuangGraduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan.
Hung-Yi WuDepartment of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan.
Ching-Dong ChangDepartment of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan.
Brent L NielsenDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, USA.
Hung-Jen LiuInstitute of Molecular Biology, National Chung Hsing University, Taichung, 402, Taiwan. hjliu5257@nchu.edu.tw.ORCID http://orcid.org/0000-0002-1460-1494
National Chung Hsing University · TWNational Pingtung University of Science and Technology · TWBrigham Young University · USMackay Junior College of Medicine, Nursing and Management · TW

Funding

Council of Agriculture 100AS-1.1.5-BQ-H3-6Ministry of Science and Technology, Taiwan MOST108-2622-B-005-001-CC2the Ministry of Education (MOE) in Taiwan 109S0023ATungs' Taichung MetroHarbor Hospital TTMHH109 R0026
6 · The paper itself

Abstract

To increase expression levels of the PCV2 Cap(d41) protein, novel baculovirus surface display vectors with multiple expression cassettes were constructed to create recombinant baculoviruses BacSC-Cap(d41), BacDD-2Cap(d41), BacDD-3Cap(d41), and BacDD-4Cap(d41). Our results reveal that the recombinant baculovirus BacDD-4Cap(d41) was able to express the highest levels of Cap(d41) protein. Optimum conditions for expressing the PCV2 Cap(d41) protein were determined, and our results show that 10

Indexed as

BaculoviridaeImmunogenicity, VaccineAnimalsCircoviridae InfectionsCircovirusGenetic VectorsMiceSpecific Pathogen-Free OrganismsSus scrofaSwineSwine DiseasesViral ProteinsViral Proteins4Cap(d41) vaccinebaculovirus surface display vectorsCap proteinCD4+ T cellscellular immune responseIFN-γPCV2virus neutralization test

Identifiers

PMID32907618
PMCPMC7487469
OpenAlexW3083771851

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.