Evidence map›Paper›PMID 32907582›Full record

ArticleMalaria journal2020

Evaluation of the colorimetric malachite green loop-mediated isothermal amplification (MG-LAMP) assay for the detection of malaria species at two different health facilities in a malaria endemic area of western Kenya.

James Gachugia, Winnie Chebore, Kephas Otieno, Caroline Wangari Ngugi, Adano Godana, Simon Kariuki

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Malaria journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Recombinase-Aided Loop-Mediated Isothermal Amplification on Human Plasmodium knowlesi.The American journal of tropical medicine and hygiene · 2024
    Article
  5. Article
  6. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

James GachugiaDepartment of Medical Microbiology, College of Health Sciences, Jomo Kenyatta University of Agriculture and Technology, P. O. Box 62000-00200, Nairobi, Kenya.
Winnie CheboreKenya Medical Research Institute, Centre for Global Health Research, P. O. Box 1578-40100, Kisumu, Kenya.
Kephas OtienoKenya Medical Research Institute, Centre for Global Health Research, P. O. Box 1578-40100, Kisumu, Kenya.
Caroline Wangari NgugiDepartment of Medical Microbiology, College of Health Sciences, Jomo Kenyatta University of Agriculture and Technology, P. O. Box 62000-00200, Nairobi, Kenya.
Adano GodanaNational Malaria Control Programme, Ministry of Health, Kenyatta National Hospital, P. O. Box, Nairobi, 19982-00202, Kenya.
Simon KariukiKenya Medical Research Institute, Centre for Global Health Research, P. O. Box 1578-40100, Kisumu, Kenya. skariuki@kemricdc.org.ORCID http://orcid.org/0000-0003-0554-1976
Kenya Medical Research Institute · KEJomo Kenyatta University of Agriculture and Technology · KEKenyatta National Hospital · KE

Funding

Kenya Medical Research Institute Internal Research Grant L317
6 · The paper itself

Abstract

backgroundPrompt diagnosis and effective malaria treatment is a key strategy in malaria control. However, the recommended diagnostic methods, microscopy and rapid diagnostic tests (RDTs), are not supported by robust quality assurance systems in endemic areas. This study compared the performance of routine RDTs and smear microscopy with a simple molecular-based colorimetric loop-mediated isothermal amplification (LAMP) at two different levels of the health care system in a malaria-endemic area of western Kenya.

methodsPatients presenting with clinical symptoms of malaria at Rota Dispensary (level 2) and Siaya County Referral Hospital (level 4) were enrolled into the study after obtaining written informed consent. Capillary blood was collected to test for malaria by RDT and microscopy at the dispensary and county hospital, and for preparation of blood smears and dried blood spots (DBS) for expert microscopy and real-time polymerase chain reaction (RT-PCR). Results of the routine diagnostic tests were compared with those of malachite green loop-mediated isothermal amplification (MG-LAMP) performed at the two facilities.

resultsA total of 264 participants were enrolled into the study. At the dispensary level, the positivity rate by RDT, expert microscopy, MG-LAMP and RT-PCR was 37%, 30%, 44% and 42%, respectively, and 42%, 43%, 57% and 43% at the county hospital. Using RT-PCR as the reference test, the sensitivity of RDT and MG-LAMP was 78.1% (CI 67.5-86.4) and 82.9% (CI 73.0-90.3) at Rota dispensary. At Siaya hospital the sensitivity of routine microscopy and MG-LAMP was 83.3% (CI 65.3-94.4) and 93.3% (CI 77.9-99.2), respectively. Compared to MG-LAMP, there were 14 false positives and 29 false negatives by RDT at Rota dispensary and 3 false positives and 13 false negatives by routine microscopy at Siaya Hospital.

conclusionMG-LAMP is more sensitive than RDTs and microscopy in the detection of malaria parasites at public health facilities and might be a useful quality control tool in resource-limited settings.

Indexed as

ColorimetryDiagnostic Tests, RoutineHumansKenyaMalaria, FalciparumMicroscopyMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPlasmodium falciparumRosaniline Dyesmalachite greenRosaniline DyesDiagnosisMalachite green loop-mediated isothermal amplificationMalariaPlasmodium

Identifiers

PMID32907582
PMCPMC7487890
OpenAlexW3084043023

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.