Evidence map›Paper›PMID 32902437›Full record

ArticleJournal of the Optical Society of America. A, Optics, image science, and vision2020

Spatio-angular fluorescence microscopy III. Constrained angular diffusion, polarized excitation, and high-NA imaging.

Talon Chandler, Hari Shroff, Rudolf Oldenbourg, Patrick La Rivière

Abstract read
In one paragraph

Article in Journal of the Optical Society of America. A, Optics, image science, and vision, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Volumetric imaging of the 3D orientation of cellular structures with a polarized fluorescence light-sheet microscope.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Talon Chandler
Hari Shroff
Rudolf Oldenbourg
Patrick La Rivière

Funding

3D imaging of cells and tissues using polarized light microscopyR35GM131843 · NIGMS · MARINE BIOLOGICAL LABORATORY · PI OLDENBOURG, RUDOLF · 2019 to 2023
$2.5M
Broadband X-ray Fluorescence Emission TomographyR01EB026300 · NIBIB · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI LA RIVIERE, PATRICK JEAN, LU, YI · 2018 to 2021
$2.4M
Instantaneous 3D imaging of cells and tissues using polarized light microscopyR01GM114274 · NIGMS · MARINE BIOLOGICAL LABORATORY · PI OLDENBOURG, RUDOLF · 2015 to 2018
$2.4M
NIBIB NIH HHS R01 EB026300NIGMS NIH HHS R01 GM114274NIGMS NIH HHS R35 GM131843
6 · The paper itself

Abstract

We investigate rotational diffusion of fluorescent molecules in angular potential wells, the excitation and subsequent emissions from these diffusing molecules, and the imaging of these emissions with high-NA aplanatic optical microscopes. Although dipole emissions only transmit six low-frequency angular components, we show that angular structured illumination can alias higher-frequency angular components into the passband of the imaging system. We show that the number of measurable angular components is limited by the relationships between three time scales: the rotational diffusion time, the fluorescence decay time, and the acquisition time. We demonstrate our model by simulating a numerical phantom in the limits of fast angular diffusion, slow angular diffusion, and weak potentials.

Identifiers

PMID32902437
PMCPMC7931634

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.