ArticleJournal of cellular biochemistry2021
Serotonin receptor type 2B activation augments TNF-α-induced matrix mineralization in murine valvular interstitial cells.
Article in Journal of cellular biochemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Specific serotonin receptor antagonist reduces murine calcific atherosclerosis.Atherosclerosis · 2026Article
- Circulating serotonin is associated with calcific aortic valve stenosis: a two-phase metabolomics study.Frontiers in pharmacology · 2026Article
- Peripheral Serotonergic Activation in Severe Aortic Stenosis: A Biochemical Perspective.International journal of molecular sciences · 2025Article
- Role of Serotonin, Membrane Transporter, and 5-HT2 Receptors in Pathogenesis of Atherosclerotic Plaque Formation in Immature Heterozygous Low-Density Lipoprotein-Receptor-Deficient Mice.International journal of molecular sciences · 2025Article
- Effects of LP533401 on vascular and bone calcification in hyperlipidemic mice.Biochemical and biophysical research communications · 2024Article
- Relationship Between Dietary Inflammatory Index and Carotid Artery Calcification in Patients with Ischemic Stroke.Journal of inflammation research · 2024Article
- Decreased serotonin transporter activity in the mitral valve contributes to progression of degenerative mitral regurgitation.Science translational medicine · 2023Article
- Indoleamine 2,3-Dioxygenase 1 Deletion-Mediated Kynurenine Insufficiency in Vascular Smooth Muscle Cells Exacerbates Arterial Calcification.Circulation · 2022Article
- Tri-Layered and Gel-Like Nanofibrous Scaffolds with Anisotropic Features for Engineering Heart Valve Leaflets.Advanced healthcare materials · 2022Article
- Serotonin-A Driver of Progressive Heart Valve Disease.Frontiers in cardiovascular medicine · 2022Review
- Biomolecules Orchestrating Cardiovascular Calcification.Biomolecules · 2021Review
- Comparison of Serotonin-Regulated Calcific Processes in Aortic and Mitral Valvular Interstitial Cells.ACS omega · 2021Article
- Pathological Insight into 5-HTInternational journal of molecular sciences · 2020Review
- Serotonin transporter deficiency in mice results in an increased susceptibility to HTR2B-dependent pro-fibrotic mechanisms in the cardiac valves and left ventricular myocardium.Cardiovascular pathology : the official journal of the Society for Cardiovascular PathologyArticle
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Authors and funding
4 authors.
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Abstract
Calcification, fibrosis, and chronic inflammation are the predominant features of calcific aortic valve disease, a life-threatening condition. Drugs that induce serotonin (5-hydroxytryptamine [5-HT]) are known to damage valves, and activated platelets, which carry peripheral serotonin, are known to promote calcific aortic valve stenosis. However, the role of 5-HT in valve leaflet pathology is not known. We tested whether serotonin mediates inflammation-induced matrix mineralization in valve cells. Real-time reverse transcription-polymerase chain reaction analysis showed that murine aortic valve interstitial cells (VICs) expressed both serotonin receptor types 2A and 2B (Htr2a and Htr2b). Although Htr2a expression was greater at baseline, Htr2b expression was induced several-fold more than Htr2a in response to the pro-calcific tumor necrosis factor-α (TNF-α) treatment. 5-HT also augmented TNF-α-induced osteoblastic differentiation and matrix mineralization of VIC, but 5-HT alone had no effects. Inhibition of serotonin receptor type 2B, using specific inhibitors or lentiviral knockdown in VIC, attenuated 5-HT effects on TNF-α-induced osteoblastic differentiation and mineralization. 5-HT treatment also augmented TNF-α-induced matrix metalloproteinase-3 expression, which was also attenuated by Htr2b knockdown. Htr2b expression in aortic roots and serum levels of peripheral 5-HT were also greater in the hyperlipidemic Apoe
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