Evidence map›Paper›PMID 32901369›Full record

ArticleDrug delivery and translational research2020

Otoliths-composed gelatin/sodium alginate scaffolds for bone regeneration.

Daisy Pereira Valido, Wilson Déda Gonçalves Júnior, Maria Eliane de Andrade, Allan Andrade Rezende, Felipe Mendes de Andrade de Carvalho, Renata de Lima, Gabriela das Graças Gomes Trindade, Caio de Alcântara Campos, Ana Maria Santos Oliveira, Eloísa Portugal Barros Silva Soares de Souza and 10 more

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Article in Drug delivery and translational research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Daisy Pereira ValidoTiradentes University, Av. Murilo Dantas, 300, Aracaju, 49010-390, Brazil.
Wilson Déda Gonçalves JúniorTiradentes University, Av. Murilo Dantas, 300, Aracaju, 49010-390, Brazil.
Maria Eliane de AndradeTiradentes University, Av. Murilo Dantas, 300, Aracaju, 49010-390, Brazil.
Allan Andrade RezendeTiradentes University, Av. Murilo Dantas, 300, Aracaju, 49010-390, Brazil.
Felipe Mendes de Andrade de CarvalhoTiradentes University, Av. Murilo Dantas, 300, Aracaju, 49010-390, Brazil.
Renata de LimaDepartment of Biotechnology, University of Sorocaba, Rodovia Raposo Tavares S/N-km 92,5, Sorocaba, SP, CEP 18023-000, Brazil.
Gabriela das Graças Gomes TrindadeDepartment of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, 49100-00, Brazil.
Caio de Alcântara CamposDepartment of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, 49100-00, Brazil.
Ana Maria Santos OliveiraDepartment of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, 49100-00, Brazil.
Eloísa Portugal Barros Silva Soares de SouzaDepartment of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, 49100-00, Brazil.
Luiza Abrahão FrankFaculty of Pharmacy, Federal University of Rio Grande do Sul, Av. Ipiranga, 2759, Porto Alegre, Rio Grande do Sul, 90610-000, Brazil.
Silvia Stanisçuaski GuterresFaculty of Pharmacy, Federal University of Rio Grande do Sul, Av. Ipiranga, 2759, Porto Alegre, Rio Grande do Sul, 90610-000, Brazil.
Eliana Midori SussuchiDepartment of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, 49100-00, Brazil.
Charlene Regina Santos MatosDepartment of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, 49100-00, Brazil.
André PolloniTiradentes University, Av. Murilo Dantas, 300, Aracaju, 49010-390, Brazil.
Adriano Antunes de Souza AraújoDepartment of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, 49100-00, Brazil.
Francine Ferreira PadilhaTiradentes University, Av. Murilo Dantas, 300, Aracaju, 49010-390, Brazil.
Patrícia SeverinoTiradentes University, Av. Murilo Dantas, 300, Aracaju, 49010-390, Brazil. patricia_severino@itp.org.br.ORCID 0000-0001-6527-6612
Eliana Barbosa SoutoFaculty of Pharmacy, University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal.
Ricardo Luiz Cavalcanti de Albuquerque JúniorTiradentes University, Av. Murilo Dantas, 300, Aracaju, 49010-390, Brazil. ricardo.patologia@uol.com.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Evidence that otoliths, mineral-rich limestone concrescences present in the inner ear of bone fishes, can accelerate bone formation in vivo has been previously reported. The goal of this work was the development, characterization, and evaluation of the cytocompatibility of otoliths-incorporated sodium alginate and gelatin scaffolds. Cynoscion acoupa-derived otoliths were characterized by X-ray fluorescence spectrometry (FRX), particle size, free lime, and weight loss by calcination. Furthermore, otoliths were incorporated into sodium alginate (ALG/OTL-s) or gelatin (GEL/OTL-s) scaffolds, previously developed by freeze-drying. Then, the scaffolds were characterized by thermogravimetric analysis (TGA/DTG), differential scanning calorimetry (DSC), infrared spectroscopy with Fourier transform (FTIR), swelling tests, and scanning electron microscopy (SEM). Cytotoxicity assays were run against J774.G8 macrophages and MC3T3-E1 osteoblasts. Data obtained from TGA/DTG, DSC, and FTIR analyses confirmed the interaction between otoliths and the polymeric scaffolds. SEM showed the homogeneous porous 3D structure rich in otolith micro-fragments in both scaffolds. Swelling of the GEL/OTL-s (63.54 ± 3.0%) was greater than of ALG/OTL-s (13.36 ± 9.9%) (p < 0.001). The viability of J774.G8 macrophages treated with both scaffolds was statistically similar to the group treated with DMEM only (p > 0.05) and significantly higher than that treated with Triton-X (p < 0.01) at 72 h. Both scaffolds showed approximately 100% growth of MC3T3-E1 osteoblasts by 24 h, similarly to control (p > 0.05). However, by 48 h, only ALG/OTL-s showed growth similar to control (p > 0.05), whereas GEL/OTL showed a significantly lower growth index (p < 0.05). In conclusion, the physicochemical profiles suggest proper interaction between the otoliths and the two developed polymeric 3D scaffolds. Moreover, both materials showed cytocompatibility with J774.G8 macrophages but the growth of MC3T3-E1 osteoblasts was higher when exposed to ALG/OTL-s. These data suggest that sodium alginate/otoliths scaffolds are potential biomaterials to be used in bone regeneration applications. Graphical abstract.

Indexed as

AlginatesBone RegenerationGelatinTissue Scaffolds3T3 CellsAnimalsBiocompatible MaterialsMiceOtolithic MembranePorositySpectroscopy, Fourier Transform InfraredTissue EngineeringAlginatesBiocompatible MaterialsGelatinBone regenerationOtolithsTissue engineeringTissue scaffolds

Identifiers

PMID32901369

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.