Discriminating larvae of two syntopic Cychramus species (Coleoptera, Nitidulidae) by means of bar-HRM analysis.

Lukas Zangl, Hannes Oberreiter, Herbert Huss, Edith Stabentheiner, Christian Sturmbauer, Stephan Koblmüller

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Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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6 authors.

Lukas ZanglInstitute of Biology, University of Graz, Universitätsplatz 2, 8010, Graz, Austria. lukas.zangl@uni-graz.at.ORCID https://orcid.org/0000-0002-1175-564X

Hannes OberreiterInstitute of Biology, University of Graz, Universitätsplatz 2, 8010, Graz, Austria.ORCID https://orcid.org/0000-0003-1019-1245

Herbert Huss, 4651, Stadl-Paura, Austria.

Edith StabentheinerInstitute of Biology, University of Graz, Schubertstraße 51, 8010, Graz, Austria.ORCID https://orcid.org/0000-0001-5228-8390

Christian SturmbauerInstitute of Biology, University of Graz, Universitätsplatz 2, 8010, Graz, Austria.ORCID https://orcid.org/0000-0003-4170-2765

Stephan KoblmüllerInstitute of Biology, University of Graz, Universitätsplatz 2, 8010, Graz, Austria. stephan.koblmueller@uni-graz.at.ORCID https://orcid.org/0000-0002-1024-3220

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Molecular genetic methods are increasingly used to supplement or substitute classical morphology-based species identification. Here, we employ a COI mini-barcode coupled high-resolution melting analysis to quickly, cost-efficiently and reliably determine larvae of two closely related Cychramus (Coleoptera, Nitidulidae) species. Euclidean distance comparison (p < 0.01) and a Welch t-test of the melting point temperatures (p < 0.01) provide highly significant statistical evidence for species specific differences in melting and fluorescence curves, thus allowing the assignment of larvae to either of the two species. This protocol serves as a fast, low-cost and low-tech method to discriminate between pairs or groups of closely related species and can be adapted and applied to various ecological research questions.

Indexed as

DNA Barcoding, TaxonomicAnimalsColeopteraLarvaSpecies SpecificityColeopteraDNA-barcodingHigh-resolution melting analysisLarvaeMini-barcodesSap beetles

Identifiers

PMID32901359

DOI10.1007/s11033-020-05786-9

PMCPMC7588358

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