ArticleProceedings of the National Academy of Sciences of the United States of America2020
SARS-CoV-2 detection using isothermal amplification and a rapid, inexpensive protocol for sample inactivation and purification.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 172 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
172 citing papers in PubMed, 1 synthesis or guideline pooled it, 293 citations in OpenAlex.
- Rapid, point-of-care antigen and molecular-based tests for diagnosis of SARS-CoV-2 infection.The Cochrane database of systematic reviews · 2021Pooled it
- Point of Care Testing and Recombinase Polymerase Amplification.Journal of clinical laboratory analysis · 2026Article
- Suppressing Primer-Driven Nonspecific Amplification in LAMP Using TrueLAMP.bioRxiv : the preprint server for biology · 2026Article
- Development of a high-copy target enhanced multiplexed crRNA-based, amplification-free detection assay (HiTECT) for Brucella spp.Molecular biology reports · 2026Article
- Extraction-Free Scalable Sample Preparation for the Rapid Detection of HIV in Blood Plasma.Analytical chemistry · 2026Article
- Heat-actuated valve implementation in a point-of-care, paper-based microfluidic device for infectious disease detection.PloS one · 2026Article
- In-Field Detection of Plant Pathogens Using Three-Dimensional-Printed Microneedles and a Portable Platform.ACS sensors · 2025Article
- Point-of-Care Extraction-Free Sample Preparation for Recombinase Polymerase Amplification of Nucleic Acid Targets in Buccal Swabs or Blood.ACS omega · 2025Article
- A lyophilized open-source RT-LAMP assay for molecular diagnostics in resource-limited settings.Life science alliance · 2025Article
- A low-cost point-of-care device for the simultaneous detection of two sexually transmitted bacterial pathogens in vaginal swab samples.The Analyst · 2025Article
- Lab-on-PCB with integrated DNA amplification and electroanalytical detection for point-of-care diagnostics.Scientific reports · 2025Article
- Colorimetric RT-LAMP for SARS-CoV-2 detection from nasopharyngeal swabs or crude saliva: a multicountry diagnostic accuracy study in Africa.The Lancet. Global health · 2025Article
- Article
- Deactivated Cas9-Engineered Magnetic Micromotors toward a Point-of-Care Digital Viral RNA Assay.ACS nano · 2025Article
- Multiplexed detection of respiratory virus RNA using optical pH sensors and injection-molded centrifugal microfluidics.Mikrochimica acta · 2025Article
- Tunable control of Cas12 activity promotes universal and fast one-pot nucleic acid detection.Nature communications · 2025Article
- A Critical Review of the CRISPR-Cas Technology in the Detection of SARS-CoV-2 Variants.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2025Review
- RUNCOV: a one-pot triplex real-time RT-LAMP as a point-of-care diagnostic tool for detecting SARS-CoV-2.Biology methods & protocols · 2025Article
- Advancements in LAMP-Based Diagnostics: Emerging Techniques and Applications in Viral Detection with a Focus on Herpesviruses in Transplant Patient Management.International journal of molecular sciences · 2024Review
- Rolosense: Mechanical Detection of SARS-CoV-2 Using a DNA-Based Motor.ACS central science · 2024Article
112 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has had an enormous impact on society worldwide, threatening the lives and livelihoods of many. The effects will continue to grow and worsen if economies begin to open without the proper precautions, including expanded diagnostic capabilities. To address this need for increased testing, we have developed a sensitive reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay compatible with current reagents, which utilizes a colorimetric readout in as little as 30 min. A rapid inactivation protocol capable of inactivating virions, as well as endogenous nucleases, was optimized to increase sensitivity and sample stability. This protocol, combined with the RT-LAMP assay, has a sensitivity of at least 50 viral RNA copies per microliter in a sample. To further increase the sensitivity, a purification protocol compatible with this inactivation method was developed. The inactivation and purification protocol, combined with the RT-LAMP assay, brings the sensitivity to at least 1 viral RNA copy per microliter in a sample. This simple inactivation and purification pipeline is inexpensive and compatible with other downstream RNA detection platforms and uses readily available reagents. It should increase the availability of SARS-CoV-2 testing as well as expand the settings in which this testing can be performed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.