ArticleAnalytical chemistry2020
Double Emulsion Picoreactors for High-Throughput Single-Cell Encapsulation and Phenotyping via FACS.
Article in Analytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- A Scalable Droplet Microfluidic Platform That Overcomes Large-Cell Constraints for Long-Term Plant Cell Culture and Embryogenesis Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Review
- Acoustic printing of conductive polymers.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Osmotically Tunable Microdroplets Enable Amplification-Free CRISPR Detection of Gene Doping.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Continuous FACS Sorting of Double Emulsion Picoreactors with a 3D-Printed Vertical Mixer.Analytical chemistry · 2025Article
- Mixed surfactants for stabilizing and controlling water transport in double emulsions.Science advances · 2025Article
- Recent Advances in Antibody Discovery Using Ultrahigh-Throughput Droplet Microfluidics: Challenges and Future Perspectives.Biosensors · 2025Review
- Continuous FACS sorting of double emulsion picoreactors with a 3D printed vertical mixer.bioRxiv : the preprint server for biology · 2025Article
- From specialization to broad adoption: Key trends in droplet microfluidic innovations enhancing accessibility to non-experts.Biomicrofluidics · 2025Article
- FACS-Sortable Triple Emulsion Picoreactors for Screening Reactions in Biphasic Environments.Advanced materials interfaces · 2025Article
- High-Throughput Single-Cell Analysis of Local Nascent Protein Deposition in 3D Microenvironments via Extracellular Protein Identification Cytometry (EPIC).Advanced materials (Deerfield Beach, Fla.) · 2025Article
- DEPICT-seq: Single-Cell Transcriptomic Analysis of Rare Cell Subsets Isolated via Nucleic Acid Cytometry.Analytical chemistry · 2024Article
- Robust Double Emulsions for Multicolor Fluorescence-Activated Cell Sorting.Analytical chemistry · 2024Article
- Review
- Unveiling the Potential of Single-Cell Encapsulation in Biomedical Applications: Current Advances and Future Perspectives.Small science · 2024Article
- Antibodies, repertoires and microdevices in antibody discovery and characterization.Lab on a chip · 2024Review
- Design automation of microfluidic single and double emulsion droplets with machine learning.Nature communications · 2024Article
- Versatility and stability optimization of flow-focusing droplet generatorsLab on a chip · 2023Article
- Flow cytometric printing of double emulsions into open droplet arrays.Lab on a chip · 2023Article
- One-Pot Droplet RT-OE-PCR for the Generation of Natively Paired Antibody Immune Libraries.Methods in molecular biology (Clifton, N.J.) · 2023Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
In the past five years, droplet microfluidic techniques have unlocked new opportunities for the high-throughput genome-wide analysis of single cells, transforming our understanding of cellular diversity and function. However, the field lacks an accessible method to screen and sort droplets based on cellular phenotype upstream of genetic analysis, particularly for large and complex cells. To meet this need, we developed Dropception, a robust, easy-to-use workflow for precise single-cell encapsulation into picoliter-scale double emulsion droplets compatible with high-throughput screening via fluorescence-activated cell sorting (FACS). We demonstrate the capabilities of this method by encapsulating five standardized mammalian cell lines of varying sizes and morphologies as well as a heterogeneous cell mixture of a whole dissociated flatworm (5-25 μm in diameter) within highly monodisperse double emulsions (35 μm in diameter). We optimize for preferential encapsulation of single cells with extremely low multiple-cell loading events (<2% of cell-containing droplets), thereby allowing direct linkage of cellular phenotype to genotype. Across all cell lines, cell loading efficiency approaches the theoretical limit with no observable bias by cell size. FACS measurements reveal the ability to discriminate empty droplets from those containing cells with good agreement to single-cell occupancies quantified via microscopy, establishing robust droplet screening at single-cell resolution. High-throughput FACS screening of cellular picoreactors has the potential to shift the landscape of single-cell droplet microfluidics by expanding the repertoire of current nucleic acid droplet assays to include functional phenotyping.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.