ArticleLife (Basel, Switzerland)2020
Potential for Applying Continuous Directed Evolution to Plant Enzymes: An Exploratory Study.
Article in Life (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Exploring the Evolutionary Landscape with Targeted In Vivo Hypermutations.Biomedicines · 2026Review
- Continuous Directed Evolution of a Short-Lived Plant Histidinol Dehydrogenase.ACS synthetic biology · 2026Article
- Ultra-efficient Integration of Gene Libraries onto Yeast Cytosolic Plasmids.ACS synthetic biology · 2025Article
- Discovery and Characterization of Mannan-Specialized GH5 Endo-1,4-β-mannanases: a Strategy for Açaí (Journal of agricultural and food chemistry · 2025Article
- Harnessing mass spectrometry-based proteomics for continuous directed evolution.Synthetic biology (Oxford, England) · 2025Article
- Fitness landscape of substrate-adaptive mutations in evolved amino acid-polyamine-organocation transporters.eLife · 2024Article
- Adapting enzymes to improve their functionality in plants: why and how.Biochemical Society transactions · 2023Review
- Multiplexed Guide RNA Expression Leads to Increased Mutation Frequency in Targeted Window Using a CRISPR-Guided Error-Prone DNA Polymerase inACS synthetic biology · 2023Article
- Directed Evolution of Aerotolerance in Sulfide-Dependent Thiazole Synthases.ACS synthetic biology · 2023Article
- Continuous Directed Evolution of a Feedback-ResistantACS synthetic biology · 2023Article
- B vitamin supply in plants and humans: the importance of vitamer homeostasis.The Plant journal : for cell and molecular biology · 2022Review
- Using continuous directed evolution to improve enzymes for plant applications.Plant physiology · 2022Article
- In vivo hypermutation and continuous evolution.Nature reviews. Methods primers · 2022Article
- Integrating continuous hypermutation with high-throughput screening for optimization of cis,cis-muconic acid production in yeast.Microbial biotechnology · 2021Article
- Structure and function of aerotolerant, multiple-turnover THI4 thiazole synthases.The Biochemical journal · 2021Article
- Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants.Plant physiology · 2021Article
- The number of catalytic cycles in an enzyme's lifetime and why it matters to metabolic engineering.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
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8 authors.
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Abstract
Plant evolution has produced enzymes that may not be optimal for maximizing yield and quality in today's agricultural environments and plant biotechnology applications. By improving enzyme performance, it should be possible to alleviate constraints on yield and quality currently imposed by kinetic properties or enzyme instability. Enzymes can be optimized more quickly than naturally possible by applying directed evolution, which entails mutating a target gene in vitro and screening or selecting the mutated gene products for the desired characteristics. Continuous directed evolution is a more efficient and scalable version that accomplishes the mutagenesis and selection steps simultaneously in vivo via error-prone replication of the target gene and coupling of the host cell's growth rate to the target gene's function. However, published continuous systems require custom plasmid assembly, and convenient multipurpose platforms are not available. We discuss two systems suitable for continuous directed evolution of enzymes, OrthoRep in
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