ReviewInternational journal of molecular sciences2020
Phylogenetic Utility of rRNA ITS2 Sequence-Structure under Functional Constraint.
Review in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Integrated ITS2 DNA Barcoding and LC-QTOF/MS Metabolomics for Authentication of Ngueak Pla Mo (Plants (Basel, Switzerland) · 2026Article
- The rps15-rpl32 intergenic locus is a phylogeographic marker for Latin American Zea mays landrace varieties and subspecies.BMC genomics · 2025Article
- Real-time dynamic monitoring and multiplex PCR identification of vector mosquitoes in Zhejiang, China.PLoS neglected tropical diseases · 2025Article
- HFTC: a hierarchical fungal taxonomic classification model for ITS sequences using low-dimensional embedding features.Frontiers in genetics · 2025Article
- A Meta-Analysis of the Human Gut Mycobiome Using Internal Transcribed Spacer Data.Microorganisms · 2024Article
- GC heterogeneity reveals sequence-structures evolution of angiosperm ITS2.BMC plant biology · 2023Article
- Compensatory Base Changes in ITS2 Secondary Structure Alignment, Modelling, and Molecular Phylogeny: An Integrated Approach to Improve Species Delimitation inJournal of fungi (Basel, Switzerland) · 2023Article
- Molecular Apomorphies in the Secondary and Tertiary Structures of Length-Variable Regions (LVRs) of 18S rRNA Shed Light on the Systematic Position of the Family Thaumastellidae (Hemiptera: Heteroptera: Pentatomoidea).International journal of molecular sciences · 2023Article
- Structure-Based GC Investigation Sheds New Light on ITS2 Evolution inInternational journal of molecular sciences · 2023Article
- Article
- Report of two distinct ribotypes in ITS sequences ofBiodiversity data journal · 2023Article
- DNA Barcoding andGenes · 2022Article
- Molecular Phylogeny, DNA Barcoding, andGenes · 2022Article
- Looking through the Lens of the Ribosome Biogenesis Evolutionary History: Possible Implications for Archaeal Phylogeny and Eukaryogenesis.Molecular biology and evolution · 2022Article
- Compensatory Base Changes and Varying Phylogenetic Effects on Angiosperm ITS2 Genetic Distances.Plants (Basel, Switzerland) · 2022Article
- Detection and Molecular Phylogenetic-Morphometric Characterization ofJournal of fungi (Basel, Switzerland) · 2022Article
- Comparison of Culturing and Metabarcoding Methods to Describe the Fungal Endophytic Assemblage ofBiology · 2021Article
- Article
- The role of internal transcribed spacer 2 secondary structures in classifying mycoparasitic Ampelomyces.PloS one · 2021Article
- Genetic and parasitological identification ofVeterinary world · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The crucial function of the internal transcribed spacer 2 (ITS2) region in ribosome biogenesis depends on its secondary and tertiary structures. Despite rapidly evolving, ITS2 is under evolutionary constraints to maintain the specific secondary structures that provide functionality. A link between function, structure and evolution could contribute an understanding to each other and recently has created a growing point of sequence-structure phylogeny of ITS2. Here we briefly review the current knowledge of ITS2 processing in ribosome biogenesis, focusing on the conservative characteristics of ITS2 secondary structure, including structure form, structural motifs, cleavage sites, and base-pair interactions. We then review the phylogenetic implications and applications of this structure information, including structure-guiding sequence alignment, base-pair mutation model, and species distinguishing. We give the rationale for why incorporating structure information into tree construction could improve reliability and accuracy, and some perspectives of bioinformatics coding that allow for a meaningful evolutionary character to be extracted. In sum, this review of the integration of function, structure and evolution of ITS2 will expand the traditional sequence-based ITS2 phylogeny and thus contributes to the tree of life. The generality of ITS2 characteristics may also inspire phylogenetic use of other similar structural regions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.