ArticleAnimal bioscience2021
Effects of using different roughages in the total mixed ration inoculated with or without coculture of Lactobacillus acidophilus and Bacillus subtilis on in vitro rumen fermentation and microbial population.
Article in Animal bioscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- Effect of dual bacterial combinations on in vitro nutrient degradability, gas production, methane emission, ruminal fermentation parameters and predictive values in sheep.Scientific reports · 2026Article
- Metabolic and metataxonomic changes in lactating holstein dairy cows during the transition from heat stress to the recovery period.Journal of animal science and technology · 2026Article
- Ensiling Time and Mixed Microbe Fermented Liquid Modulate In Vitro Digestibility and Rumen Fermentation of Fermented Total Mixed Rations.Veterinary sciences · 2025Article
- Effects of quadric probiotic blends on rumen fermentation, nutrient degradability, and methane emission in sheep: an in vitro study.AMB Express · 2025Article
- Probiotic supplementation in sustainable sheep production: impacts on health, performance, and methane mitigation.Tropical animal health and production · 2025Review
- Relationship between pyrimidines, purines, and fatty acids in milk of dairy cows fed distinct carbohydrate types: A metabolomic approach.JDS communications · 2025Article
- Effects of Italian ryegrass silage-based total mixed ration on rumen fermentation, growth performance, blood metabolites, and bacterial communities of growing Hanwoo heifers.Journal of animal science and technology · 2023Article
- Article
- Kimchi cabbage (Journal of animal science and technology · 2021Article
- Seasonal Influence on Rumen Microbiota, Rumen Fermentation, and Enteric Methane Emissions of Holstein and Jersey Steers under the Same Total Mixed Ration.Animals : an open access journal from MDPI · 2021Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveThis study aimed to determine the effects of different roughages in total mixed ration (TMR) inoculated with or without coculture of Lactobacillus acidophilus (L. acidophilus) and Bacillus subtilis (B. subtilis) on in vitro rumen fermentation and microbial population.
methodsThree TMRs formulations composed of different forages were used and each TMR was grouped into two treatments: non-fermented TMR and fermented TMR (F-TMR) (inoculated with coculture of L. acidophilus and B. subtilis). After fermentation, the fermentation, chemical and microbial profile of the TMRs were determined. The treatments were used for in vitro rumen fermentation to determine total gas production, pH, ammonianitrogen (NH3-N), and volatile fatty acids (VFA). Microbial populations were determined by quantitative real-time polymerase chain reaction (PCR). All data were analyzed as a 3×2 factorial arrangement design using the MIXED procedure of Statistical Analysis Systems.
resultsChanges in the fermentation (pH, lactate, acetate, propionate, and NH3-N) and chemical composition (moisture, crude protein, crude fiber, and ash) were observed. For in vitro rumen fermentation, lower rumen pH, higher acetate, propionate, and total VFA content were observed in the F-TMR group after 24 h incubation (p<0.05). F-TMR group had higher acetate concentration compared with the non-fermented group. Total VFA was highest (p<0.05) in F-TMR containing combined forage of domestic and imported source (F-CF) and F-TMR containing Italian ryegrass silage and corn silage (F-IRS-CS) than that of TMR diet containing oat, timothy, and alfalfa hay. The microbial population was not affected by the different TMR diets.
conclusionThe use of Italian ryegrass silage and corn silage, as well as the inoculation of coculture of L. acidophilus and B. subtilis, in the TMR caused changes in the pH, lactate and acetate concentrations, and chemical composition of experimental diets. In addition, F-TMR composed with Italian ryegrass silage and corn silage altered ruminal pH and VFA concentrations during in vitro rumen fermentation experiment.
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