ArticleJournal of experimental botany2020
Soybean TILLING-by-Sequencing+ reveals the role of novel GmSACPD members in unsaturated fatty acid biosynthesis while maintaining healthy nodules.
Article in Journal of experimental botany, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Fatty Acid Desaturases GmROD1s Are Involved in Nodulation by Regulating the Flux of Polyunsaturated Fatty Acids.Plant, cell & environment · 2026Article
- The Effect of Selenium-Arabinogalactan Nanocomposite on Fatty Acid Composition in Soybean Seedlings Grown fromPlants (Basel, Switzerland) · 2026Article
- Discovery of two tightly linked soybean genes at the qSCN10 (O) locus conferring broad-spectrum resistance to soybean cyst nematode.Communications biology · 2025Article
- Improving Soybean Seed Sucrose Content using TILLING by Sequencing Analyses of The Soybean Sucrose Synthase Gene Family.Frontiers in plant science · 2025Article
- Regulation of seed traits in soybean.aBIOTECH · 2023Review
- Soybean genetics, genomics, and breeding for improving nutritional value and reducing antinutritional traits in food and feed.The plant genome · 2023Review
- A novel type of Brassica napus with higher stearic acid in seeds developed through genome editing of BnaSAD2 family.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Soybean gene co-expression network analysis identifies two co-regulated gene modules associated with nodule formation and development.Molecular plant pathology · 2023Article
- Bioengineering of Soybean Oil and Its Impact on Agronomic Traits.International journal of molecular sciences · 2023Review
- Analysis of Delta(9) fatty acid desaturase gene family and their role in oleic acid accumulation inFrontiers in plant science · 2023Article
- Progress in soybean functional genomics over the past decade.Plant biotechnology journal · 2022Review
- Genome-wide identification and analysis of soybean acyl-ACP thioesterase gene family reveals the role of GmFAT to improve fatty acid composition in soybean seed.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- The Soybean High Density 'Forrest' by 'Williams 82' SNP-Based Genetic Linkage Map Identifies QTL and Candidate Genes for Seed Isoflavone Content.Plants (Basel, Switzerland) · 2021Article
- SlRCM1, which encodes tomato Lutescent1, is required for chlorophyll synthesis and chloroplast development in fruits.Horticulture research · 2021Article
- TILLING-by-SequencingCells · 2021Article
- TILLING-by-SequencingInternational journal of molecular sciences · 2021Article
- Improving soybean seed oil without poor agronomics.Journal of experimental botany · 2020Article
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15 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Developing soybean lines with high levels of stearic acid is a primary goal of the soybean industry. Most high-stearic-acid soybeans carry different GmSACPD-C mutated alleles. However, due to the dual role of GmSACPD-C in seeds and nodule development, all derived deleterious GmSACPD-C mutant alleles are of extremely poor agronomic value because of defective nodulation. The soybean stearoyl-acyl carrier protein desaturase (GmSACPD) gene family is composed of five members. Comparative genomics analysis indicated that SACPD genes were duplicated and derived from a common ancestor that is still present in chlorophytic algae. Synteny analysis showed the presence of segment duplications between GmSACPD-A/GmSACPD-B, and GmSACPD-C/GmSACPD-D. GmSACPD-E was not contained in any duplicated segment and may be the result of tandem duplication. We developed a TILLING by Target Capture Sequencing (Tilling-by-Sequencing+) technology, a versatile extension of the conventional TILLING by sequencing, and successfully identified 12, 14, and 18 ethyl methanesulfonate mutants at the GmSACPD-A, GmSACPD-B, and GmSACPD-D genes, respectively. Functional analysis of all identified mutants revealed an unprecedented role of GmSACPD-A, GmSACPD-B, and GmSACPD-D in unsaturated fatty acid biosynthesis without affecting nodule development and structure. This discovery will positively impact the development of high-stearic-acid lines to enhance soybean nutritional value without potential developmental tradeoffs.
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