ArticleJournal of the American Society for Mass Spectrometry2020
Storage Conditions of Human Kidney Tissue Sections Affect Spatial Lipidomics Analysis Reproducibility.
Article in Journal of the American Society for Mass Spectrometry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
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- Imaging and spatially resolved mass spectrometry applications in nephrology.Nature reviews. Nephrology · 2025Review
- Spatial Multiomics Toward Understanding Neurological Systems.Journal of mass spectrometry : JMS · 2025Review
- Morphological and molecular preservation through universal preparation of fresh-frozen tissue samples for multimodal imaging workflows.Nature protocols · 2024Review
- Frontiers in Mass Spectrometry-Based Spatial Metabolomics: Current Applications and Challenges in the Context of Biomedical Research.Trends in analytical chemistry : TRAC · 2024Article
- Recent Analytical Methodologies in Lipid Analysis.International journal of molecular sciences · 2024Review
- Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling.Nature protocols · 2023Review
- How to sample a seizure plant: the role of the visualization spatial distribution analysis ofForensic sciences research · 2023Article
- Pre-analytical sample handling standardization for reliable measurement of metabolites and lipids in LC-MS-based clinical research.Journal of mass spectrometry and advances in the clinical lab · 2023Article
- Sample preparation optimization of insects and zebrafish for whole-body mass spectrometry imaging.Analytical and bioanalytical chemistry · 2022Article
- A reference tissue atlas for the human kidney.Science advances · 2022Article
- Metal-Assisted Laser Desorption Ionization Imaging Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Uncovering Molecular Heterogeneity in the Kidney With Spatially Targeted Mass Spectrometry.Frontiers in physiology · 2022Review
- A novel experimental workflow to determine the impact of storage parameters on the mass spectrometric profiling and assessment of representative phosphatidylethanolamine lipids in mouse tissues.Analytical and bioanalytical chemistry · 2021Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Lipids often are labile, unstable, and tend to degrade overtime, so it is of the upmost importance to study these molecules in their most native state. We sought to understand the optimal storage conditions for spatial lipidomic analysis of human kidney tissue sections. Specifically, we evaluated human kidney tissue sections on several different days throughout the span of a week using our established protocol for elucidating lipids using high mass resolution matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). We studied kidney tissue sections stored under five different conditions: open stored at -80 °C, vacuumed sealed and stored at -80 °C, with matrix preapplied before storage at -80 °C, under a nitrogen atmosphere and stored at -80 °C, and at room temperature in a desiccator. Results were compared to data obtained from kidney tissue sections that were prepared and analyzed immediately after cryosectioning. Data was processed using METASPACE. After a week of storage, the sections stored at room temperature showed the largest amount of lipid degradation, while sections stored under nitrogen and at -80 °C retained the greatest number of overlapping annotations in relation to freshly cut tissue. Overall, we found that molecular degradation of the tissue sections was unavoidable over time, regardless of storage conditions, but storing tissue sections in an inert gas at low temperatures can curtail molecular degradation within tissue sections.
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Registered trials
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