Evidence map›Paper›PMID 32897500›Full record

ArticleClinical oral investigations2021

Bisphosphonate-related osteonecrosis induced change in alveolar bone architecture in rats with participation of Wnt signaling.

Vanessa Costa de Sousa Ferreira, Amanda Pimentel Lopes, Nicholas Militão Alves, Fatima Regina Nunes Sousa, Karuza Maria Alves Pereira, Delane Viana Gondim, Vírginia Claúdia Carneiro Girão, Renata Ferreira Carvalho Leitão, Paula Goes

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In one paragraph

Article in Clinical oral investigations, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.3field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 32 citations in OpenAlex.

  1. Article
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  3. Antiresorptive effect of a semisynthetic triterpene from Combretum leprosum Mart. on dexamethasone-induced osteoporosis in rats.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Vanessa Costa de Sousa FerreiraDepartment of Morphology, Medical School, Federal University of Ceará, Fortaleza, CE, Brazil.
Amanda Pimentel LopesDepartment of Morphology, Medical School, Federal University of Ceará, Fortaleza, CE, Brazil.
Nicholas Militão AlvesDepartment of Morphology, Medical School, Federal University of Ceará, Fortaleza, CE, Brazil.
Fatima Regina Nunes SousaDepartment of Morphology, Medical School, Federal University of Ceará, Fortaleza, CE, Brazil.
Karuza Maria Alves PereiraDepartment of Morphology, Medical School, Federal University of Ceará, Fortaleza, CE, Brazil.
Delane Viana GondimDepartment of Morphology, Medical School, Federal University of Ceará, Fortaleza, CE, Brazil.
Vírginia Claúdia Carneiro GirãoDepartment of Morphology, Medical School, Federal University of Ceará, Fortaleza, CE, Brazil.
Renata Ferreira Carvalho LeitãoDepartment of Morphology, Medical School, Federal University of Ceará, Fortaleza, CE, Brazil.
Paula GoesNucleus of Study and Research in Pain, Inflammation and Osteoimmunology (NEPDIO), Medical School, Federal University of Ceará, Fortaleza, CE, Brazil. paulagpinheiro@yahoo.com.br.ORCID http://orcid.org/0000-0002-9704-2412
Universidade Federal do Ceará · BR

Funding

CNPq 423303/2016-3.
6 · The paper itself

Abstract

objectiveThis work aimed to study the role of inflammation in medication-related osteonecrosis of the jaw (MRONJ) in rats with focus on Wnt signaling.

methodsA total of 36 female Wistar rats (12 weeks ± 200 g) were divided into 2 groups (n = 6) in 3 experiments: saline (SAL) and zoledronic acid (ZOL). For MRONJ induction, rats received 0.1 mg/kg of ZOL (ip) 3×/week for 9 weeks. Animals from the SAL group received 0.1 mg/kg of 0.9% SAL, ip 3×/week for 9 weeks. On the 8th week, 3 left upper molars were extracted, and on the 11th week, they were euthanized. Maxillae were evaluated by macroscopic and histopathological analyses; scanning electron microscopy (SEM); immunohistochemistry for DKK-1, Wnt 10b, and caspase-3; and Raman spectrometry. Gingiva was also collected for TNF-α e IL-1β quantification.

resultsBone necrosis was confirmed by healing impairment, reduced number of viable osteocytes, increased caspase-3 immunoexpression, and increased number of empty lacunae (p < 0.05). ZOL enhanced inflammation and increased gingival levels of IL-1β and TNF-α (p < 0.05). Irregular indentations were seen on bone after ZOL administration. Bone necrosis was marked by reduced amount of total and type I collagen. ZOL reduced the mineral/matrix ratio and increased carbonate/phosphate ratio. It was observed a significant reduction on Wnt10b and beta-catenin immunolabeling in the bone tissue of ZOL group.

conclusionIn summary, MRONJ model caused bone necrosis due to intense inflammation. Wnt signaling seems to play an important role in this process. CLINICAL RELEVANCE: New therapeutic strategies focusing on Wnt pathway can provide an interesting approach for future treatments.

Indexed as

Bisphosphonate-Associated Osteonecrosis of the JawBone Density Conservation AgentsAnimalsDiphosphonatesFemaleMaxillaRatsRats, WistarWnt Signaling PathwayZoledronic AcidBone Density Conservation AgentsDiphosphonatesZoledronic AcidBisphosphonatesBone architectureInflammationOsteonecrosisWnt signalingZoledronic acid

Identifiers

PMID32897500
OpenAlexW3084052763

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.