ArticleJournal of pharmaceutical and biomedical analysis2020
Untargeted metabolomics profiling and hemoglobin normalization for archived newborn dried blood spots from a refrigerated biorepository.
Article in Journal of pharmaceutical and biomedical analysis, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Phthalates measured at birth and risk of testicular cancer in adolescents and young adults.JNCI cancer spectrum · 2026Article
- Maternal red blood cell folate metabolites were dynamically associated with neonatal amino acids and acylcarnitines in heel blood: a prospective cohort study.Nutrition & metabolism · 2026Article
- Molecular Reactivity in Maternal Pregnancy Blood and Neonatal Dried Blood Spots Is Associated with the Risk of Pediatric Acute Lymphoblastic Leukemia.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2025Article
- Postpartum metabolomics profile predicts 8-years postdelivery women's subclinical atherosclerosis measures.Scientific reports · 2025Article
- Active Molecular Network Discovery Links Lifestyle Variables to Breast Cancer in the Long Island Breast Cancer Study Project.Environment & health (Washington, D.C.) · 2024Article
- Effects of storage temperature and time on metabolite profiles measured in dried blood spots, dried blood microsamplers, and plasma.The Science of the total environment · 2024Article
- Dried Blood Spots in Neonatal Studies: A Computational Analysis for the Role of the Hematocrit Effect.Pharmaceuticals (Basel, Switzerland) · 2023Article
- A global metabolomics minefield: Confounding effects of preanalytical factors when studying rare disorders.Analytical science advances · 2023Article
- Associations between the Gut Microbiota, Urinary Metabolites, and Diet in Women during the Third Trimester of Pregnancy.Current developments in nutrition · 2023Article
- Article
- Advancing Exposomic Research in Prenatal Respiratory Disease Programming.Immunology and allergy clinics of North America · 2023Review
- Comprehensive lipidomic profiling by plasma separation cards.Analytical and bioanalytical chemistry · 2023Article
- Molecular Gatekeeper Discovery: Workflow for Linking Multiple Exposure Biomarkers to Metabolomics.Environmental science & technology · 2022Article
- Comparison of untargeted and targeted perfluoroalkyl acids measured in adolescent girls.Chemosphere · 2022Article
- Review
- Lead exposure and serum metabolite profiles in pregnant women in Mexico City.Environmental health : a global access science source · 2021Article
- Tooth biomarkers to characterize the temporal dynamics of the fetal and early-life exposome.Environment international · 2021Article
- Interdisciplinary data science to advance environmental health research and improve birth outcomes.Environmental research · 2021Article
- Low-dose exposure of glyphosate-based herbicides disrupt the urine metabolome and its interaction with gut microbiota.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Archived dried blood spots (DBS) following newborn screening are an attractive resource for interrogating early-life biology using untargeted metabolomics. Therefore, they have the potential to substantially aid etiological studies, particularly for rare and low-frequency childhood diseases and disorders. However, metabolite quantification in DBS is hindered by variation sources not present in serum and plasma samples such as the hematocrit effect and unknown initial blood volumes. Hemoglobin (Hb) is an appropriate correlate for hematocrit in experimentally-generated DBS punches. However, since many biorepositories worldwide archive DBS at 4-5 °C, there is a need to validate the utility of Hb for DBS archived under refrigeration. We evaluated two simple spectroscopic methods for measuring Hb in DBS stored at 4 +/- 2 °C for up to 21 years, obtained from the newborn screening program at the Karolinska University Hospital, Sweden. Spearman correlation analysis and Akaike Information Criterion model selection found that measurement of a Hb sodium lauryl sulfate complex at 540 nm better described nuisance variation than Hb measured at 404 nm, or using age of spot alone. This is the first study to profile metabolites and to propose a normalization factor for metabolite measurements from DBS archived for decades at 4 °C.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.