ArticleEMBO reports2020
Mitochondrial respiration is required to provide amino acids during fermentative proliferation of fission yeast.
Article in EMBO reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 48 citations in OpenAlex.
- Methods to Study Mitochondrial Metabolism and Homeostasis in Fission Yeast.Yeast (Chichester, England) · 2026Review
- PomBase in 2026: expanding knowledge, modeling connections.Genetics · 2026Review
- The role of mitotype variation and positive epistasis in trait differences between Saccharomyces species.Genetics · 2026Article
- Heterochromatin epimutations impose mitochondrial dysfunction to confer antifungal resistance.The EMBO journal · 2026Article
- Article
- Can Unicellular Organisms Sequester a Germline? The Yeast-Germline Hypothesis.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Development of tolerance to bedaquiline by overexpression of trypanosomal acetate: succinate CoA transferase in Mycobacterium smegmatis.Communications biology · 2025Article
- The Key Enzymes of Carbon Metabolism and the Glutathione Antioxidant System ProtectJournal of fungi (Basel, Switzerland) · 2024Article
- InFrontiers in fungal biology · 2024Article
- Membrane transporters in cell physiology, cancer metabolism and drug response.Disease models & mechanisms · 2023Review
- Cell cycle-linked vacuolar pH dynamics regulate amino acid homeostasis and cell growth.Nature metabolism · 2023Article
- A coarse-grained resource allocation model of carbon and nitrogen metabolism in unicellular microbes.Journal of the Royal Society, Interface · 2023Article
- Genetic effects on molecular network states explain complex traits.Molecular systems biology · 2023Article
- Multifactor transcriptional control of alternative oxidase induction integrates diverse environmental inputs to enable fungal virulence.Nature communications · 2023Article
- Optimization of energy production and central carbon metabolism in a non-respiring eukaryote.Current biology : CB · 2023Article
- Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils.Journal of visualized experiments : JoVE · 2023Article
- Kinetic and Regulatory Properties ofInternational journal of molecular sciences · 2023Article
- Elevated energy costs of biomass production in mitochondrial respiration-deficient Saccharomyces cerevisia.FEMS yeast research · 2023Article
- Role of amino acid metabolism in mitochondrial homeostasis.Frontiers in cell and developmental biology · 2023Review
- Genome-wide quantification of contributions to sexual fitness identifies genes required for spore viability and health in fission yeast.PLoS genetics · 2022Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
When glucose is available, many organisms repress mitochondrial respiration in favour of aerobic glycolysis, or fermentation in yeast, that suffices for ATP production. Fission yeast cells, however, rely partially on respiration for rapid proliferation under fermentative conditions. Here, we determined the limiting factors that require respiratory function during fermentation. When inhibiting the electron transport chain, supplementation with arginine was necessary and sufficient to restore rapid proliferation. Accordingly, a systematic screen for mutants growing poorly without arginine identified mutants defective in mitochondrial oxidative metabolism. Genetic or pharmacological inhibition of respiration triggered a drop in intracellular levels of arginine and amino acids derived from the Krebs cycle metabolite alpha-ketoglutarate: glutamine, lysine and glutamic acid. Conversion of arginine into these amino acids was required for rapid proliferation when blocking the respiratory chain. The respiratory block triggered an immediate gene expression response diagnostic of TOR inhibition, which was muted by arginine supplementation or without the AMPK-activating kinase Ssp1. The TOR-controlled proteins featured biased composition of amino acids reflecting their shortage after respiratory inhibition. We conclude that respiration supports rapid proliferation in fermenting fission yeast cells by boosting the supply of Krebs cycle-derived amino acids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.