ArticleImmune network2020
Effects of Cryopreservation and Thawing on Single-Cell Transcriptomes of Human T Cells.
Article in Immune network, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Tissue-resident immune cells drive genetic risk in autoimmune and lung diseases.Nature immunology · 2026Article
- DUSP1 is a key driver of disease persistence and potential therapeutic target in hairy cell leukemia.Blood advances · 2026Article
- Caveola and Its Resident Proteins, Caveolin-1 and -2, Play Critical Roles in the Pathogenesis of nAMD and PCV.Investigative ophthalmology & visual science · 2026Article
- Developmental chronology of mouse embryo from 2-cell stage through birth.Nature cell biology · 2026Article
- Distinct T Cell Dysregulation Reflects Disease Severity and Progression in Infantile Epileptic Spasms Syndrome and Lennox-Gastaut Syndrome.Immune network · 2025Article
- Single-cell transcriptome conservation in a multispecies comparative analysis of fresh and cryopreserved insulinoma cell lines.Veterinary oncology (London, England) · 2025Article
- Directly selecting cell-type marker genes for single-cell clustering analyses.Cell reports methods · 2024Article
- Enabling methanol fixation of pediatric nasal wash during respiratory illness for single cell sequencing in comparison with fresh samples.Pediatric research · 2024Article
- Single-Cell RNA-Sequencing of RVFV Infection.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Post-thaw application of ROCK-inhibitors increases cryopreserved T-cell yield.RSC medicinal chemistry · 2023Article
- Profiling of mature-stage human breast milk cells identifies six unique lactocyte subpopulations.Science advances · 2022Article
- Comprehensive evaluation of the effects of long-term cryopreservation on peripheral blood mononuclear cells using flow cytometry.BMC immunology · 2022Article
- Reestablishment of spermatogenesis after more than 20 years of cryopreservation of rat spermatogonial stem cells reveals an important impact in differentiation capacity.PLoS biology · 2022Article
- Understanding the Transcriptomic Landscape to Drive New Innovations in Musculoskeletal Regenerative Medicine.Current osteoporosis reports · 2022Review
- Tumor-specific T cells support chemokine-driven spatial organization of intratumoral immune microaggregates needed for long survival.Journal for immunotherapy of cancer · 2022Article
- Cryopreservation of human cancers conserves tumour heterogeneity for single-cell multi-omics analysis.Genome medicine · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryopreservation and thawing of PBMCs are inevitable processes in expanding the scale of experiments in human immunology. Here, we carried out a fundamental study to investigate the detailed effects of PBMC cryopreservation and thawing on transcriptomes. We sorted Tregs from fresh and cryopreserved/thawed PBMCs from an identical donor and performed single-cell RNA-sequencing (scRNA-seq). We found that the cryopreservation and thawing process minimally affects the key molecular features of Tregs, including
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