Evidence map›Paper›PMID 32895448›Full record

ArticleScientific reports2020

Spliced genes in muscle from Nelore Cattle and their association with carcass and meat quality.

Danielly B S Silva, Larissa F S Fonseca, Daniel G Pinheiro, Ana F B Magalhães, Maria M M Muniz, Jesus A Ferro, Fernando Baldi, Luis A L Chardulo, Robert D Schnabel, Jeremy F Taylor and 1 more

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
2.6field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 46 citations in OpenAlex.

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  10. Transcriptomic analysis of yakAnimal biotechnology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Danielly B S SilvaSchool of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil. daniellyberaldo@gmail.com.ORCID http://orcid.org/0000-0002-3144-7476
Larissa F S FonsecaSchool of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil.
Daniel G PinheiroSchool of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil.
Ana F B MagalhãesSchool of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil.
Maria M M MunizSchool of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil.
Jesus A FerroSchool of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil.
Fernando BaldiSchool of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil.
Luis A L CharduloSchool of Veterinary and Animal Science, São Paulo State University (UNESP), Botucatu, SP, Brazil.
Robert D SchnabelDivision of Animal Sciences, University of Missouri Columbia, Columbia, MO, USA.ORCID http://orcid.org/0000-0001-5018-7641
Jeremy F TaylorDivision of Animal Sciences, University of Missouri Columbia, Columbia, MO, USA.ORCID http://orcid.org/0000-0002-6997-7245
Lucia G AlbuquerqueSchool of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil. lgalb@fcav.unesp.br.
Universidade Estadual Paulista (Unesp) · BRNational Council for Scientific and Technological Development · BRUniversity of Missouri · USNúcleo de Pesquisas Aplicadas (Brazil) · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Transcript data obtained by RNA-Seq were used to identify differentially expressed alternatively spliced genes in ribeye muscle tissue between Nelore cattle that differed in their ribeye area (REA) or intramuscular fat content (IF). A total of 166 alternatively spliced transcripts from 125 genes were significantly differentially expressed in ribeye muscle between the highest and lowest REA groups (p ≤ 0.05). For animals selected on their IF content, 269 alternatively spliced transcripts from 219 genes were differentially expressed in ribeye muscle between the highest and lowest IF animals. Cassette exons and alternative 3' splice sites were the most frequently found alternatively spliced transcripts for REA and IF content. For both traits, some differentially expressed alternatively spliced transcripts belonged to myosin and myotilin gene families. The hub transcripts were identified for REA (LRRFIP1, RCAN1 and RHOBTB1) and IF (TRIP12, HSPE1 and MAP2K6) have an important role to play in muscle cell degradation, development and motility. In general, transcripts were found for both traits with biological process GO terms that were involved in pathways related to protein ubiquitination, muscle differentiation, lipids and hormonal systems. Our results reinforce the biological importance of these known processes but also reveal new insights into the complexity of the whole cell muscle mRNA of Nelore cattle.

Indexed as

Alternative SplicingRed MeatTranscriptomeAnimalsCattleFood QualityMicrofilament ProteinsMuscle ProteinsMusclesRNA, MessengerMicrofilament ProteinsMuscle ProteinsRNA, Messenger

Identifiers

PMID32895448
PMCPMC7477197
OpenAlexW3083717533

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.