Evidence map›Paper›PMID 32895447›Full record

ArticleScientific reports2020

Quantitative proteomic analysis of the tizoxanide effect in vero cells.

K A Yamamoto, K Blackburn, E Migowski, M B Goshe, D T Brown, D F Ferreira, M R Soares

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

K A YamamotoDepartment of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Caixa Postal 68563, Rio de Janeiro, RJ, 21941-909, Brazil.ORCID http://orcid.org/0000-0002-1618-9325
K BlackburnDepartment of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC, USA.
E MigowskiInstitute of Pediatrics and Puericulture Martagão Gesteira, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.ORCID http://orcid.org/0000-0003-4372-4255
M B GosheDepartment of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC, USA.ORCID http://orcid.org/0000-0003-0845-9943
D T BrownDepartment of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC, USA.ORCID http://orcid.org/0000-0002-2650-9070
D F FerreiraDepartment of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC, USA.ORCID http://orcid.org/0000-0001-9449-4862
M R SoaresDepartment of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Caixa Postal 68563, Rio de Janeiro, RJ, 21941-909, Brazil. marcia@iq.ufrj.br.ORCID http://orcid.org/0000-0001-9272-9488
Universidade Federal do Rio de Janeiro · BRNorth Carolina State University · US

Funding

National Science Foundation (NSF) DBI-1126244
6 · The paper itself

Abstract

Nitazoxanide (NTZ) is effective against helminths and numerous microorganisms, including bacteria and viruses. In vivo, NTZ is metabolized into Tizoxanide (TIZ), which is the active circulating metabolite. With the emergence of SARS-Cov-2 as a Pandemic agent, NTZ became one of the molecules already approved for human use to engage clinical trials, due to results in vitro showing that NTZ was highly effective against the SARS-Cov-2, agent of COVID-19. There are currently several ongoing clinical trials mainly in the USA and Brazil involving NTZ due not only to the in vitro results, but also for its long-known safety. Here, we study the response of Vero cells to TIZ treatment and unveil possible mechanisms for its antimicrobial effect, using a label-free proteomic approach (LC/MS/MS) analysis to compare the proteomic profile between untreated- and TIZ-treated cells. Fifteen differentially expressed proteins were observed related to various biological processes, including translation, intracellular trafficking, RNA processing and modification, and signal transduction. The broad antimicrobial range of TIZ points towards its overall effect in lowering cell metabolism and RNA processing and modification. The decreased levels of FASN, HNRNPH and HNRNPK with the treatment appear to be important for antiviral activity.

Indexed as

AnimalsAnti-Infective AgentsChlorocebus aethiopsFatty Acid SynthasesProteomeRibonucleoproteinsThiazolesVero CellsAnti-Infective AgentsFatty Acid SynthasesProteomeRibonucleoproteinsThiazolestizoxanide

Identifiers

PMID32895447
PMCPMC7477200
OpenAlexW3083536777

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.