ArticlePlant & cell physiology2020
Identification of a Unique Type of Isoflavone O-Methyltransferase, GmIOMT1, Based on Multi-Omics Analysis of Soybean under Biotic Stress.
Article in Plant & cell physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.
- Multi-omics revolution to promote plant breeding efficiency.Frontiers in plant science · 2022Pooled it
- Discovery of pseudobaptigenin synthase in fungal-infected Trifolium pratense roots.The Plant journal : for cell and molecular biology · 2026Article
- The role of coumarin biosynthesis in modulating plant drought responses: insights from PpBMT overexpression in Arabidopsis.BMC plant biology · 2026Article
- Discovery of an isoflavone biosynthetic pathway in Iridaceae plants.Nature communications · 2026Article
- Identification of an isoflavone 6-hydroxylase involved in tectorigenin biosynthesis in kudzu (Plant biotechnology (Tokyo, Japan) · 2026Article
- Integrated Multi-Omics Analysis Revealed the Synergistic Regulatory Mechanisms of Salt Tolerance in Soybean (Kefeng 1).Plants (Basel, Switzerland) · 2026Article
- Sugarcane Breeding in the Genomic Era: Integrative Strategies and Emerging Technologies.Plants (Basel, Switzerland) · 2026Review
- Reciprocal BLUP: A Predictability-Guided Multi-Omics Framework for Plant Phenotype Prediction.Plants (Basel, Switzerland) · 2025Article
- Lonicera caerulea genome reveals molecular mechanisms of freezing tolerance and anthocyanin biosynthesis.Journal of advanced research · 2025Article
- Omics-based identification of the broader effects of 2-hydroxyisoflavanone synthase gene editing on a gene regulatory network beyond isoflavonoid loss in soybean hairy roots.Plant & cell physiology · 2025Article
- Metabolome Profiling and Predictive Modeling of Dark Green Leaf Trait in Bunching Onion Varieties.Metabolites · 2025Article
- CYP71D8 and CYP82A2 catalyze the last committed step in biosynthesis of glyceollin isomers in soybean.Plant biotechnology (Tokyo, Japan) · 2025Article
- An R2R3-type MYB transcription factor, GmMYB77, negatively regulates isoflavone accumulation in soybean [Glycine max (L.) Merr.].Plant biotechnology journal · 2025Article
- Multiomics-based assessment of the impact of airflow on diverse plant callus cultures.Scientific data · 2025Article
- Proteomics: An Essential Tool to Study Plant-Specialized Metabolism.Biomolecules · 2024Review
- Elucidating the metabolic roles of isoflavone synthase-mediated protein-protein interactions in yeast.bioRxiv : the preprint server for biology · 2024Article
- Methylation Modification in Ornamental Plants: Impact on Floral Aroma and Color.International journal of molecular sciences · 2024Review
- A chromosome-level genome reveals genome evolution and molecular basis of anthraquinone biosynthesis in Rheum palmatum.BMC plant biology · 2024Article
- Neofunctionalization of anProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Identification of Genes Responsible for the Synthesis of Glycitein Isoflavones in Soybean Seeds.Plants (Basel, Switzerland) · 2024Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isoflavonoids are commonly found in leguminous plants. Glycitein is one of the isoflavones produced by soybean. The genes encoding the enzymes in the isoflavone biosynthetic pathway have mostly been identified and characterized. However, the gene(s) for isoflavone O-methyltransferase (IOMT), which catalyzes the last step of glycitein biosynthesis, has not yet been identified. In this study, we conducted multi-omics analyses of fungal-inoculated soybean and indicated that glycitein biosynthesis was induced in response to biotic stress. Moreover, we identified a unique type of IOMT, which participates in glycitein biosynthesis. Soybean seedlings were inoculated with Aspergillus oryzae or Rhizopus oligosporus and sampled daily for 8 d. Multi-omics analyses were conducted using liquid chromatography-tandem mass spectrometry and RNA sequencing. Metabolome analysis revealed that glycitein derivatives increased following fungal inoculation. Transcriptome co-expression analysis identified two candidate IOMTs that were co-expressed with the gene encoding flavonoid 6-hydroxylase (F6H), the key enzyme in glycitein biosynthesis. The enzymatic assay of the two IOMTs using respective recombinant proteins showed that one IOMT, named as GmIOMT1, produced glycitein. Unlike other IOMTs, GmIOMT1 belongs to the cation-dependent OMT family and exhibited the highest activity with Zn2+ among cations tested. Moreover, we demonstrated that GmIOMT1 overexpression increased the levels of glycitein derivatives in soybean hairy roots when F6H was co-expressed. These results strongly suggest that GmIOMT1 participates in inducing glycitein biosynthesis in response to biotic stress.
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